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Team:Bielefeld-Germany/Results/Characterization
From 2010.igem.org
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===<partinfo>P1010</partinfo>: ''ccdB''-gene=== | ===<partinfo>P1010</partinfo>: ''ccdB''-gene=== | ||
We transformed this BioBrick into ''Escherichia coli'' JM109, DH5α, TOP10 and DB3.1. ''E. coli'' JM109 and DH5α seem to be ''ccdB'' resistant because there were as much colonies after P1010 transformation as observed with DB3.1. The P1010 works as expected in ''E. coli'' TOP10 (no colonies after transformation). | We transformed this BioBrick into ''Escherichia coli'' JM109, DH5α, TOP10 and DB3.1. ''E. coli'' JM109 and DH5α seem to be ''ccdB'' resistant because there were as much colonies after P1010 transformation as observed with DB3.1. The P1010 works as expected in ''E. coli'' TOP10 (no colonies after transformation). | ||
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| + | ==Sequenced BioBricks== | ||
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| + | ===Own BioBricks=== | ||
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| + | The sequencing of the following of our own BioBricks was succesful and lead to the expected results: | ||
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| + | * <partinfo>K389002</partinfo> | ||
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| + | * <partinfo>K389003</partinfo> | ||
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| + | * <partinfo>K389004</partinfo> | ||
| + | |||
| + | * <partinfo>K389005</partinfo> | ||
Revision as of 14:13, 24 August 2010
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Contents |
Tested BioBricks
We tested the following BioBricks:
<partinfo>K238008</partinfo>: virA
We made a restriction analysis and sequenced parts of this BioBrick. There were problems - more information within a short time.
<partinfo>BBa_K238011</partinfo>: vir-promotor
We made a restriction analysis and sequenced parts of this BioBrick.
<partinfo>P1010</partinfo>: ccdB-gene
We transformed this BioBrick into Escherichia coli JM109, DH5α, TOP10 and DB3.1. E. coli JM109 and DH5α seem to be ccdB resistant because there were as much colonies after P1010 transformation as observed with DB3.1. The P1010 works as expected in E. coli TOP10 (no colonies after transformation).
Sequenced BioBricks
Own BioBricks
The sequencing of the following of our own BioBricks was succesful and lead to the expected results:
- <partinfo>K389002</partinfo>
- <partinfo>K389003</partinfo>
- <partinfo>K389004</partinfo>
- <partinfo>K389005</partinfo>
