Team:Bielefeld-Germany/Results/Characterization

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===<partinfo>P1010</partinfo>: ''ccdB''-gene===
===<partinfo>P1010</partinfo>: ''ccdB''-gene===
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We transformed this BioBrick into ''Escherichia coli'' JM109, DH5α, TOP10 and DB3.1. ''E. coli'' JM109 and DH5α seem to be ''ccdB'' resistant because there were as much colonies after P1010 transformation as observed with TOP10. The P1010 works as expected in ''E. coli'' TOP10 (no colonies after transformation).
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We transformed this BioBrick into ''Escherichia coli'' JM109, DH5α, TOP10 and DB3.1. ''E. coli'' JM109 and DH5α seem to be ''ccdB'' resistant because there were as much colonies after P1010 transformation as observed with DB3.1. The P1010 works as expected in ''E. coli'' TOP10 (no colonies after transformation).

Revision as of 11:51, 17 August 2010

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Contents

Tested BioBricks

We tested the following BioBricks:

<partinfo>K238008</partinfo>: virA

We made a restriction analysis and sequenced parts of this BioBrick. There were problems - more information within a short time.


<partinfo>BBa_K238011</partinfo>: vir-promotor

We made a restriction analysis and sequenced parts of this BioBrick.


<partinfo>P1010</partinfo>: ccdB-gene

We transformed this BioBrick into Escherichia coli JM109, DH5α, TOP10 and DB3.1. E. coli JM109 and DH5α seem to be ccdB resistant because there were as much colonies after P1010 transformation as observed with DB3.1. The P1010 works as expected in E. coli TOP10 (no colonies after transformation).

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