Team:INSA-Lyon/Protocols/Digestio
From 2010.igem.org
(Difference between revisions)
m |
m |
||
(3 intermediate revisions not shown) | |||
Line 5: | Line 5: | ||
{{INSA-Lyon/menugauche}} | {{INSA-Lyon/menugauche}} | ||
+ | {{INSA-Lyon/menunotebook}} | ||
<html xmlns="http://www.w3.org/1999/xhtml" dir="ltr" lang="en-US" xml:lang="en"> | <html xmlns="http://www.w3.org/1999/xhtml" dir="ltr" lang="en-US" xml:lang="en"> | ||
- | < | + | <style type="text/css";> |
- | + | .firstHeading{background-image: url(https://static.igem.org/mediawiki/2010/9/93/Team_INSA-Lyon_header_DroppyColi.jpg); backgroung-repeat: no repeat;} | |
- | + | ul#topnav li:hover { | |
- | + | background: #f200d0 url("https://static.igem.org/mediawiki/2010/d/d9/Team_INSA-Lyon_bouton_fushia.gif") repeat-x; | |
- | + | } | |
- | + | ul#topnav li span { background: #f200d0;} | |
- | + | </style> | |
- | + | ||
- | </ | + | |
+ | <script type="text/javascript"> | ||
+ | $(document).ready(function() { | ||
- | + | $("ul#topnav li").hover(function() { //Hover over event on list item | |
- | + | $(this).css({ 'background' : '#f200d0 url(https://static.igem.org/mediawiki/2010/d/d9/Team_INSA-Lyon_bouton_fushia.gif) repeat-x'}); //Add background color and image on hovered list item | |
- | + | $(this).find("span").show(); //Show the subnav | |
+ | } , function() { //on hover out... | ||
+ | $(this).css({ 'background' : 'none'}); //Ditch the background | ||
+ | $(this).find("span").hide(); //Hide the subnav | ||
+ | }); | ||
- | + | }); | |
- | + | </script> | |
- | + | ||
- | </ | + | |
- | <div id=" | + | |
+ | <div id="corps2"> | ||
<h3> Protocols </h3> | <h3> Protocols </h3> | ||
- | + | <br><p>Choose a protocol to read its description : | |
+ | <br> | ||
+ | <br></p> | ||
</html> | </html> | ||
{{Template:INSALyon2010_Protocole}} | {{Template:INSALyon2010_Protocole}} | ||
Line 43: | Line 49: | ||
<style type="text/css"> | <style type="text/css"> | ||
- | #list_extra{list-style-type: | + | #list_extra{list-style-type:circle;} |
</style> | </style> | ||
Latest revision as of 21:09, 25 October 2010
Protocols
Choose a protocol to read its description :
- Competent cells
- Transformation
- DNA extraction
- Digestion
- Ligation
- Measure of temperature and shaking speed influence
- Measure of osmotic pressure influence
- Granules extraction and intein cleavage
- Biofilms quantification
- Extra
Digestion
Single digestion :
- Add x µL DNA (0,5 to 1 ug)
- Add 15 µL DNA extracted from the part transformation.
- Add 2 µL 10X buffer
- Add the enzyme last
- Add 0,5 to 2 µL restriction enzyme (max 1/10 of final volume)
- Add sterile water qs 20 µL
- Incubate 1h at 37°C.
Double digestion EcoRI/SpeI:
- Add x µL DNA (0,5 to 1 µg). Add 15 µL DNA extracted from the part transformation.
- Add 2 µL buffer Tango 10x
- Add 1 µL SpeI
- Add sterile water qs 20 µL
- Incubate 1h at 37°C
- Add 2 µL buffer Tango 10x
- Add 1 µL EcoRI
- Incubate 1h at 37°C
Double digestion EcoRI/XbaI :
- Add x µL DNA. Add 15 µL DNA extracted from the part transformation.
- Add 4 µL buffer Tango 10x
- Add 1 µL XbaI
- Add 0,5 µL EcoRI
- Add sterile water qs 20 µL
- Incubate 1h at 37°C
Double digestion XbaI/PstI :
- Add x µL DNA. Add 15 µL DNA extracted from the part transformation.
- Add 2 µL buffer Tango 10x
- Add 1µL XbaI
- Add 1 µL PstI
- Add sterile water qs 20 µL
- Incubate 1h at 37°C
Double digestion XbaI/SpeI :
- Add x µL DNA. Add 15 µL DNA extracted from the part transformation.
- Add 2 µL buffer Tango 10x
- Add 1 µL XbaI
- Add 1 µL SpeI
- Add sterile water qs 20 µL
- Incubate 1h at 37°C