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Team:INSA-Lyon/Project/Stage2/Results
From 2010.igem.org
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<h2>Results</h2> | <h2>Results</h2> | ||
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| - | <br><h3><font color="purple">Observation of the | + | <br><h3><font color="purple">Observation of the phasin-intein fusion protein (BBa_K342002)on the granule</font></h3><br><br> |
<p>Our ligation didn't work out so we couldn't realize the observations as planned. | <p>Our ligation didn't work out so we couldn't realize the observations as planned. | ||
We had many difficulties to add the constitutive promoter despite numerous tries.</p> <br><br> | We had many difficulties to add the constitutive promoter despite numerous tries.</p> <br><br> | ||
Revision as of 22:54, 27 October 2010
Results
Observation of the phasin-intein fusion protein (BBa_K342002)on the granule
Our ligation didn't work out so we couldn't realize the observations as planned. We had many difficulties to add the constitutive promoter despite numerous tries.
Lipidic inclusion in the PHB drop
We made a double transformation with pILI1 and 20N: The resultant bacteria synthesize the PHB drops and over synthesize the licopen.
When we had obtained the clones, we started four different series of cultures in order to analyzed the will of the lipid when the granule are produced.
- Serie 1 : in LB media with glucose 7% and 2,5µg of RedNile
- pILI1 with ampiciline
- double transformation with ampiciline
- double transformation with ampiciline and kanamycine
- The lipid part with kanamycine
- Serie 2 : in LB media with 2,5µg of RedNile
- pILI1 with ampiciline
- double transformation with ampiciline
- double transformation with ampiciline and kanamycine
- The lipid part with kanamycine
- Serie 3 : in LB media with glucose 7%
- pILI1 with ampiciline
- double transformation with ampiciline and kanamycine
- The lipid part with kanamycine
- Serie 4 : in LB media, the double transformation with ampiciline and kanamycine
We observed a sample of each culture under fluorescence microscope. Unfortunately we couldn't see the difference between the granules and the licopen.
