Team:INSA-Lyon/Project/Stage2/Results

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Results


Observation of the part BBa_K342002 on the granule



Our ligation didn't work out so we couldn't realize the observations as planned. We had many difficulties to add the constitutive promoter despite numerous tries.




Lipidic inclusion in the PHB drop



We made a double transformation with pILI1 and 20N: The resultant bacteria synthesize the PHB drops and over synthesize the licopen.

When we had obtained the clones, we started four different series of cultures in order to analyzed the will of the lipid when the granule are produced.

  1. Serie 1 : in LB media with glucose 7% and 2,5µg of RedNile
    1. pILI1 with ampiciline
    2. double transformation with ampiciline
    3. double transformation with ampiciline and kanamycine
    4. The lipid part with kanamycine

  2. Serie 2 : in LB media with 2,5µg of RedNile
    1. pILI1 with ampiciline
    2. double transformation with ampiciline
    3. double transformation with ampiciline and kanamycine
    4. The lipid part with kanamycine

  3. Serie 3 : in LB media with glucose 7%
    1. pILI1 with ampiciline
    2. double transformation with ampiciline and kanamycine
    3. The lipid part with kanamycine

  4. Serie 4 : in LB media, the double transformation with ampiciline and kanamycine

We observed a sample of each culture under fluorescence microscope. Unfortunately we couldn't see the difference between the granules and the licopen.


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