User contributions

From 2010.igem.org

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• 21:35, 23 October 2010 (diff | hist) N Minnesota-experimental/29 April 2010 ‎ (New page: '''Tony''' PCR purification on overnight digests of Eut C and Eut G from 4/28. 25 microliter ligation reaction of pucmod BB and Eut C/G GFP. Ligated at room temperature.)
• 21:33, 23 October 2010 (diff | hist) Minnesota-experimental/28 April 2010 ‎
• 21:30, 23 October 2010 (diff | hist) N Minnesota-experimental/28 April 2010 ‎ (New page: '''Tony and Ian''' Preparation of samples for SDS-Page.)
• 21:15, 23 October 2010 (diff | hist) N Minnesota-experimental/27 April 2010 ‎ (New page: '''Annie''' Minipreps of previous day inoculations. '''Ian''' Nanodrop of minipreps. Preparation of minipreps for DNA sequencing at University of Minnesota BioMedical Genomics Center. PC...)
• 21:12, 23 October 2010 (diff | hist) N Minnesota-experimental/26 April 2010 ‎ (New page: '''Rachel''' Inoculated pMN and LK overnight at thirty seven degrees Celsius. Negative control of pucmodBB GFP.)
• 21:09, 23 October 2010 (diff | hist) N Minnesota-experimental/23 April 2010 ‎ (New page: '''Tony and Ian''' Ran digestion reactions out on gel to confirm band sizes. Transformations of JM109 with pLKMNS vector. Plated out fifty microliters and placed in thirty seven degree i...)
• 21:07, 23 October 2010 (diff | hist) N Minnesota-experimental/21 April 2010 ‎ (New page: '''Annie''' Minipreps of overnight cultures. Digestion reactions of minipreps with EcoRI/PstI. Stored overnight in thirty seven degree Celsius incubator.)
• 21:04, 23 October 2010 (diff | hist) N Minnesota-experimental/20 April 2010 ‎ (New page: '''Matt''' Overnight cultures of pucmod BB LK, MN, and S. Six of pMNLKS, one of pMN, one of pS, one of pLK, and one of pucBB GFP. Total of 10 tubes overnight at thirty seven degrees Cels...)
• 21:03, 23 October 2010 (diff | hist) Minnesota-experimental/19 April 2010 ‎
• 21:02, 23 October 2010 (diff | hist) N Minnesota-experimental/19 April 2010 ‎ (New page: '''Annie''' Transformations of Eut LK, MN, and S. Stored overnight at thirty seven degrees Celsius.)
• 21:01, 23 October 2010 (diff | hist) N Minnesota-experimental/16 April 2010 ‎ (New page: '''Ian''' Ran gel of digests and purified digests. Set up ligations of of pMNLK.)
• 20:58, 23 October 2010 (diff | hist) Minnesota-experimental/15 April 2010 ‎
• 20:56, 23 October 2010 (diff | hist) N Minnesota-experimental/15 April 2010 ‎ (New page: '''Rachel''' Ran gel of plasmid digests at 90 volts.)
• 20:55, 23 October 2010 (diff | hist) N Minnesota-experimental/14 April 2010 ‎ (New page: '''Ian'' Gel electrophoresis of previous day PCR reactions. Digestion of plasmids and pLK. Stored at thirty seven degrees Celsius overnight.)
• 20:53, 23 October 2010 (diff | hist) Minnesota-experimental/13 April 2010 ‎
• 20:53, 23 October 2010 (diff | hist) N Minnesota-experimental/13 April 2010 ‎ (New page: '''Annie''' Minipreps of pLK and MN insert.)
• 20:52, 23 October 2010 (diff | hist) N Minnesota-experimental/12 April 2010 ‎ (New page: '''Rachel''' Inoculation of ligations pLK and MN insert overnight at thirty seven degrees Celsius.)
• 20:51, 23 October 2010 (diff | hist) N Minnesota-experimental/9 April 2010 ‎ (New page: '''Annie''' Transformations of previous day ligations. Plated 10 microliters of each transformation. Plates stored overnight at thirty-seven degrees Celsius.)
• 20:49, 23 October 2010 (diff | hist) N Minnesota-experimental/8 April 2010 ‎ (New page: '''Tony''' Ran out Eut LK and MN digests on gel and cut out DNA bands. '''Ian''' Gel purification of insert and backbone. Ligation stored at room temperature.)
• 20:30, 23 October 2010 (diff | hist) N Minnesota-experimental/7 April 2010 ‎ (New page: '''Tony''' Minipreps of Eut LK, MN, and S. Eluted in 80 microliters buffer EB. Two fifty microliter digestion reactions (EcoRI/SpeI and EcoRI/XbaI) of each of three genes (Eut LK, MN, an...)
• 19:49, 23 October 2010 (diff | hist) N Minnesota-experimental/5 April 2010 ‎ (New page: '''Rachel''' Made ampicillin/LB media plates.)
• 19:48, 23 October 2010 (diff | hist) N Minnesota-experimental/29 March 2010 ‎ (New page: '''Ian''' Ran out gels of previous day digestions.)
• 19:48, 23 October 2010 (diff | hist) N Minnesota-experimental/28 March 2010 ‎ (New page: '''Tony''' Plasmid preps of samples from 3/27. Eut LK, MN, S in pucmod BB. Set up digestions of EcoRI/SpeI and EcoRI/XbaI.)
• 19:46, 23 October 2010 (diff | hist) N Minnesota-experimental/27 March 2010 ‎ (New page: '''Tony''' Digestions run out on gel. Gel failure causes new overnight cultures to be prepared.)
• 19:45, 23 October 2010 (diff | hist) N Minnesota-experimental/26 March 2010 ‎ (New page: '''Tony''' Plasmid mini-preps of liquid cultures of Eut LK, Eut MN, Eut S. Each miniprep deigested with EcoRI/XbaI and EcoRI/SpeI. Digestions set at 37 degrees Celsius overnight.)
• 19:44, 23 October 2010 (diff | hist) N Minnesota-experimental/25 March 2010 ‎ (New page: '''Ian''' Sent Eut S, Eut LK, and Eut MN for sequencing at University of Minnesota BioMedical Genomics Center.)
• 19:43, 23 October 2010 (diff | hist) N Minnesota-experimental/24 March 2010 ‎ (New page: '''Ian''' Gel electrophoresis of plasmid digest (pucmod/Eut X with BglII/NotI).)
• 19:41, 23 October 2010 (diff | hist) N Minnesota-experimental/9 March 2010 ‎ (New page: '''Annie''' Minipreps with elution buffer 50 microliters EB. PCR and gel electrophoresis of minipreps. Results indicated presence of Eut S gene. '''Ian''' Gel electrophoresis of plasmid ...)
• 19:40, 23 October 2010 (diff | hist) N Minnesota-experimental/8 March 2010 ‎ (New page: '''Annie''' Overnight culture of six colonies in LB media in 4 microliters of ampicillin. Stored at 37 degrees Celsius. '''Tony and Ian''' Plasmid digest of plasmid preps using BglII/Not ...)
• 19:38, 23 October 2010 (diff | hist) N Minnesota-experimental/6 March 2010 ‎ (New page: '''Tony and Ian''' Minipreps of Eut LK and Eut MN. Eluted in 50 microliters buffer EB.)
• 19:37, 23 October 2010 (diff | hist) N Minnesota-experimental/5 March 2010 ‎ (New page: '''Tony''' Cultivated pucmod BB/Eut LK colonies in three LB tubes with 5 mL ampicillin. Stored at 37 degrees Celsius. '''Ian''' Cultivated pucmod BB/Eut MN colonies in LB with ampicillin ...)
• 19:36, 23 October 2010 (diff | hist) N Minnesota-experimental/4 March 2010 ‎ (New page: '''Tony''' Purification of digestion and ligation (pucmod BB) with PCR purification kit. Ligation reaction performed (25 microliters) of pucmod BB and Eut LK. '''Matt''' Transformation of...)
• 19:34, 23 October 2010 (diff | hist) N Minnesota-experimental/2 March 2010 ‎ (New page: '''Rachel''' Used PCR purification kit to purify digestion reaction. Performed ligation reaction with pucmod BB BglII/NotI vector and Eut MN BglII/NotI insert. Reaction was a 25 microlit...)
• 19:32, 23 October 2010 (diff | hist) N Minnesota-experimental/1 March 2010 ‎ (New page: '''Rachel''' Concentrated Eut MN solution using speed-vac for thirty minutes. Ran digest of gel purified Eut MN PCR product with BglII and NotI in a 50 microliter reaction. Incubated ove...)
• 19:31, 23 October 2010 (diff | hist) N Minnesota-experimental/28 February 2010 ‎ (New page: '''Tony and Ian''' Ran PCR product from Eut MN and Eut LK PCR out on gel. Fragments of LK cut out and purified using two columns with 30 microliters EB each. Fragments of MN cut out usin...)
• 19:30, 23 October 2010 (diff | hist) N Minnesota-experimental/26 February 2010 ‎ (New page: '''Tony and Ian''' PCR amplification of Eut MN and Eut LK genes. Four fifty microliter reactions.)
• 19:29, 23 October 2010 (diff | hist) N Minnesota-experimental/25 February 2010 ‎ (New page: '''Rachel''' Minipreps of pucmod BB EGFP overnight cultures.)
• 19:28, 23 October 2010 (diff | hist) N Minnesota-experimental/24 February 2010 ‎ (New page: '''Tony''' Cultivation of pucmod BB EGFP colonies. Overnight cultures of five colonies.)
• 19:21, 23 October 2010 (diff | hist) N Minnesota-experimental/19 February 2010 ‎ (2/19/2010)
• 19:19, 23 October 2010 (diff | hist) N Minnesota-experimental/17 February 2010 ‎ (New page: '''Tony''' Transformation of ligation mixture (psb3K5 and DsRed) into JM109 with both LB and Kan. Transformation plated overnight. Set up overnight cultures of previous transformation. S...)
• 19:19, 23 October 2010 (diff | hist) N Minnesota-experimental/16 February 2010 ‎ (New page: '''Tony''' Ligation reaction failed. Digestion products (dsRed and psb3K5) ligated together in a 25 microliter reaction.)
• 19:18, 23 October 2010 (diff | hist) N Minnesota-experimental/11 February 2010 ‎ (New page: '''Annie''' Transformation and plating of ligated cells. 2 plates left at 37 degrees Celsius. '''Tony''' Digestion from 2/10 run out on 1 percent agarose gel at 100 volts for fifty minute...)
• 19:15, 23 October 2010 (diff | hist) N Minnesota-experimental/10 February 2010 ‎ (New page: '''Tony''' Purified gel elutions of psb3K5BB EGFP (BglI/NotI) and DsRed Express 2 (BglI/NotI) Set up 25 microliter ligation reaction with psb3K5BB EGFP and DsRed Express 2. Enzymes used w...)
• 19:13, 23 October 2010 (diff | hist) N Minnesota-experimental/9 February 2010 ‎ (New page: '''Rachel''' Minipreps DNA Purification of overnight cultures.)
• 19:12, 23 October 2010 (diff | hist) Minnesota-experimental/8 February 2010 ‎
• 19:11, 23 October 2010 (diff | hist) N Minnesota-experimental/8 February 2010 ‎ (New page: Preparation of overnight cultures of DsRed Express 2 and psb3K5BB. Placed overnight at 37 degrees Celsius.)
• 19:11, 23 October 2010 (diff | hist) Minnesota-experimental/4 February 2010 ‎
• 19:10, 23 October 2010 (diff | hist) Minnesota-experimental/4 February 2010 ‎
• 19:06, 23 October 2010 (diff | hist) N Minnesota-experimental/1 February 2010 ‎ (New page: Initial iGEM meeting. Initial discussion of project, concept, competition, and expectations for the program. We also had beginning discussions of possible ideas for the project. We left...)
• 17:07, 21 October 2010 (diff | hist) N Minnesota-experimental/4 February 2010 ‎ (New page: Event)

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