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From 2010.igem.org

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Rachel Concentrated Eut MN solution using speed-vac for thirty minutes. Ran digest of gel purified Eut MN PCR product with BglII and NotI in a 50 microliter reaction. Incubated overnight at thirty-seven degrees Celsius.

Annie Ran PCR reaction of Eut S gene. Eut S gene digested with BglII/NotI. Eut S and Eut S digest run out on gel.