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From 2010.igem.org

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Rachel Used PCR purification kit to purify digestion reaction. Performed ligation reaction with pucmod BB BglII/NotI vector and Eut MN BglII/NotI insert. Reaction was a 25 microliter reaction.

Tony and Matt Concentrated Eut LK solution in speed-vac for sixteen minutes. Eut LK solution digested with BglII/NotI (50 microliter reaction).

Rachel and Ian Transformation of 15 microliters of ligation reaction from 3/2. Plated on amp plates at 37 degree overnight.