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From 2010.igem.org

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Annie Transformation and plating of ligated cells. 2 plates left at 37 degrees Celsius.

Tony Digestion from 2/10 run out on 1 percent agarose gel at 100 volts for fifty minutes.

Rachel Spun down cells at 400 rpm for 5 minutes. Removal of 380 microliters following centrifuge.