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From 2010.igem.org

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Tony Purification of digestion and ligation (pucmod BB) with PCR purification kit. Ligation reaction performed (25 microliters) of pucmod BB and Eut LK.

Matt Transformation of Eut LK/pucmod BB; stored in 37 degree incubator.

Ian Second attempt at transformation of Eut LK/pucmod BB; stored in 37 degree incubator.