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From 2010.igem.org

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Tony Purified gel elutions of psb3K5BB EGFP (BglI/NotI) and DsRed Express 2 (BglI/NotI) Set up 25 microliter ligation reaction with psb3K5BB EGFP and DsRed Express 2. Enzymes used were BglI and NotI Set up digestion reaction of psb3K5sBBEGFP with enzymes NotI/BglII and PstI/EcoRi.