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Team:HokkaidoU Japan/NotebookTest
From 2010.igem.org
since 13 Jul 2010
since 1 Aug 2010
Calendar
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Abstract
Monday, July 12, 2010
- The first experiment for us, transformation with BioBrick Devices.
Wednesday, July 21, 2010
- Preparation of competent cells
- Single colony isolation of E. coli which had been transformed on Monday, July 12, 2010
Monday, July 26, 2010
- Cultivation of transformed E. coli for the next day's miniprep
Tuesday, July 27, 2010
- Miniprep
Monday, August 2, 2010
- Restriction enzyme digestion of plasmids which had been purified by miniprep
- Electrophoresis assay
Tuesday, August 3, 2010
Wednesday, August 4, 2010
Thursday, August 5, 2010
Friday, August 6, 2010
Monday, August 9, 2010
- Initial amplification of the BioBrick parts (BBa_I14032 "2-11P", BBa_F2621 "2-21H", BBa_K098995 "3-1E" and BBa_E1010 "1-18F") that are used in the construction of concentration-sensitive and heat-sensitive E. coli
- This is a preliminary experiment before starting the main project
Tuesday, August 10, 2010
- PCR amplification of the other parts (BBa_F1610, BBa_B0034, BBa_B0015 and BBa_K098995) and electrophoresis for confirmation
Wednesday, August 11, 2010
- Preparation for the next day's miniprep
- Estimation of enzyme activity
- Preparation of the glycerol stocks
Thursday, August 12, 2010
- Miniprep
- Electrophoresis analysis of the DNA
