Team:HokkaidoU Japan/Notebook/September10


3 piece ligation, continued

  • Yesterday after purification by gel extraction electrophoresis showed very weak bands
  • This time increased PCR cycles to 40
  • Everything else was same as last time
  • But again it was a failure
  • Thought that template went bad,so used PCR Product as template
  • Extension was 90 sec, did 30 cycles