Team:HokkaidoU Japan/Notebook/August20


Amplified vector by PCR

Electrophoresis to confirm amplification

Amplified pSB1C3, pSB1T3, pSB1A3 accordingly to the table bellow.

Reagent Amount
DW  34 uL
10x buffer  5 uL
2mM dNTP  5 uL
25mM MgSO4  3 uL
Suffix-F  0.5 uL
Prefix-R  0.5 uL
KOD  1 uL
DNA Template  1 uL
Total  50 uL
  • pSB1T3 failed to amplify