Electrophoresis of pSB1C3 concentrated to 50 ug/uL

• Added 2.8 uL of 6x SB to 17.4 uL of pSB1C3 solution digested yesterday and electrophoresed

Lane	DNA
１	Added too much of marker, mistake
２	λ/Hind III & EcoR I
３	pSB1C3 solution
４	pSB1C3 solution

• IF digestion and ligation went well there should be bands of dimers, trimers but none of the were visible
• Only band visible was monomer（about 2000 bp）

Filtration of pSB1A3, pSB1C3 and pSB1K3 PCR solutions

Remaining amount from check via electrophoresis，namely 49 uL was filtrated with Microcon YM-10