Electrophoresis 1

Electrophoresis of yesterdays ligation of PCR products

• Checked the result of yesterdays ligation of PCR products by electrophoresis
• Added 4 uL of 6x Sample Buffer to make 12 uL in total
• Used 20 uL of EtOH

Mini prep

• Used cells incubated for 18h in LB broth
  • 1-18F didn't grow well
• Centrifuge after adding 430 uL of chloroform、collected supernatant
• Added 2-propanol to 1-18F, but after centrifugation there were no precipitation
  • Melted in 30 uL of TE and did electrophoresis

Electrophoresis 2

Take 1

Take 2 after few minutes

Checked plasmids gathered via mini prep and PCR products, by electrophoresis.

Because we wont do restriction enzyme digestion reaction system is ass follows.

• Used markers are λ/HindIII and pUC119/HinfI
• Marker in lane 1 (λ/HindIII) leaked out
• Mini prep sample，1-18F band in lane 3 wasn't visible so we decided PCR it tomorrow
• In lane 4 there were some visible plasmid dimers and trimer s