From 2010.igem.org
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| <p>Agarose gel electrophoresis --> good insert for L1 et L2</p> | | <p>Agarose gel electrophoresis --> good insert for L1 et L2</p> |
| <p>Ligation L3: 10 µL of L2 , 5µL of L1 in 20µL<br><br></p> | | <p>Ligation L3: 10 µL of L2 , 5µL of L1 in 20µL<br><br></p> |
| + | |
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| + | <p>Transformation of the ligation 1F/24A </p> |
| + | <p>Extraction of DNA: 1F, 24A, 14K </p> |
| + | <p>Agarose gel electrophoresis: ok except 14K whose the band do not have the size expected !? |
| + | <p>Digestion: 1F by S+P, 24A by X+P |
| + | <p>Agarose gel electrophoresis: 1F-->oK,24A: not digested (we forgot to centrifugate after adding the enzymes)</p> |
| + | |
| <br><br><br><br><br> | | <br><br><br><br><br> |
| </html> | | </html> |
Revision as of 15:11, 31 August 2010
July 27
Extraction of L1 in 40µL
Digestion of 5 colonies of L2 by X+P
Gel electrophoresis -->1 colony of L2 has the good insert (L2G)
Digestion of L1 by S+P and E+P and digestion of L2G by X+P
Agarose gel electrophoresis --> good insert for L1 et L2
Ligation L3: 10 µL of L2 , 5µL of L1 in 20µL
Transformation of the ligation 1F/24A
Extraction of DNA: 1F, 24A, 14K
Agarose gel electrophoresis: ok except 14K whose the band do not have the size expected !?
Digestion: 1F by S+P, 24A by X+P
Agarose gel electrophoresis: 1F-->oK,24A: not digested (we forgot to centrifugate after adding the enzymes)
June
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| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/4_June_2010&action=edit 4]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/5_June_2010&action=edit 5]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/6_June_2010&action=edit 6]
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[http://2010.igem.org/wiki/index.php?title=INSA-Lyon/7_June_2010&action=edit 7]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/8_June_2010&action=edit 8]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/9_June_2010&action=edit 9]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/10_June_2010&action=edit 10]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/11_June_2010&action=edit 11]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/12_June_2010&action=edit 12]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/13_June_2010&action=edit 13]
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[http://2010.igem.org/wiki/index.php?title=INSA-Lyon/14_June_2010&action=edit 14]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/15_June_2010&action=edit 15]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/16_June_2010&action=edit 16]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/17_June_2010&action=edit 17]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/18_June_2010&action=edit 18]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/19_June_2010&action=edit 19]
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[http://2010.igem.org/wiki/index.php?title=INSA-Lyon/21_June_2010&action=edit 21]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/22_June_2010&action=edit 22]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/23_June_2010&action=edit 23]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/24_June_2010&action=edit 24]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/25_June_2010&action=edit 25]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/26_June_2010&action=edit 26]
| [http://2010.igem.org/INSA-Lyon/27_June_2010 27]
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[http://2010.igem.org/INSA-Lyon/28_June_2010 28]
| [http://2010.igem.org/INSA-Lyon/29_June_2010 29]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/30_June_2010&action=edit 30]
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July
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| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/1_July_2010&action=edit 1]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/2_July_2010&action=edit 2]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/3_July_2010&action=edit 3]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/4_July_2010&action=edit 4]
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[http://2010.igem.org/wiki/index.php?title=INSA-Lyon/5_July_2010&action=edit 5]
| [http://2010.igem.org/INSA-Lyon/6_July_2010 6]
| [http://2010.igem.org/INSA-Lyon/7_July_2010 7]
| [http://2010.igem.org/INSA-Lyon/8_July_2010 8]
| [http://2010.igem.org/INSA-Lyon/9_July_2010 9]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/10_July_2010&action=edit 10]
| [http://2010.igem.org/INSA-Lyon/11_July_2010 11]
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[http://2010.igem.org/INSA-Lyon/12_July_2010 12]
| [http://2010.igem.org/INSA-Lyon/13_July_2010 13]
| [http://2010.igem.org/INSA-Lyon/14_July_2010 14]
| [http://2010.igem.org/INSA-Lyon/15_July_2010 15]
| [http://2010.igem.org/INSA-Lyon/16_July_2010 16]
| [http://2010.igem.org/INSA-Lyon/17_July_2010 17]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/18_July_2010&action=edit 18]
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[http://2010.igem.org/INSA-Lyon/19_July_2010 19]
| [http://2010.igem.org/INSA-Lyon/20_July_2010 20]
| [http://2010.igem.org/INSA-Lyon/21_July_2010 21]
| [http://2010.igem.org/INSA-Lyon/22_July_2010 22]
| [http://2010.igem.org/INSA-Lyon/23_July_2010 23]
| [http://2010.igem.org/INSA-Lyon/24_July_2010 24]
| [http://2010.igem.org/INSA-Lyon/25_July_2010 25]
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[http://2010.igem.org/INSA-Lyon/26_July_2010 26]
| [http://2010.igem.org/INSA-Lyon/27_July_2010 27]
| [http://2010.igem.org/INSA-Lyon/28_July_2010 28]
| [http://2010.igem.org/INSA-Lyon/29_July_2010 29]
| [http://2010.igem.org/INSA-Lyon/30_July_2010 30]
| [http://2010.igem.org/INSA-Lyon/31_July_2010 31]
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August
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[http://2010.igem.org/INSA-Lyon/2_August_2010 2]
| [http://2010.igem.org/INSA-Lyon/3_August_2010 3]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/4_August_2010&action=edit 4]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/5_August_2010&action=edit 5]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/6_August_2010&action=edit 6]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/7_August_2010&action=edit 7]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/8_August_2010&action=edit 8]
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[http://2010.igem.org/wiki/index.php?title=INSA-Lyon/9_August_2010&action=edit 9]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/10_August_2010&action=edit 10]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/11_August_2010&action=edit 11]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/12_August_2010&action=edit 12]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/13_August_2010&action=edit 13]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/14_August_2010&action=edit 14]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/15_August_2010&action=edit 15]
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[http://2010.igem.org/INSA-Lyon/16_August_2010 16]
| [http://2010.igem.org/INSA-Lyon/17_August_2010 17]
| [http://2010.igem.org/INSA-Lyon/18_August_2010 18]
| [http://2010.igem.org/INSA-Lyon/19_August_2010 19]
| [http://2010.igem.org/INSA-Lyon/20_August_2010 20]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/21_August_2010&action=edit 21]
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[http://2010.igem.org/INSA-Lyon/23_August_2010 23]
| [http://2010.igem.org/INSA-Lyon/24_August_2010 24]
| [http://2010.igem.org/INSA-Lyon/25_August_2010 25]
| [http://2010.igem.org/INSA-Lyon/26_August_2010 26]
| [http://2010.igem.org/INSA-Lyon/27_August_2010 27]
| [http://2010.igem.org/INSA-Lyon/28_August_2010 28]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/29_August_2010&action=edit 29]
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[http://2010.igem.org/INSA-Lyon/30_August_2010 30]
| [http://2010.igem.org/INSA-Lyon/31_August_2010 31]
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September
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| [http://2010.igem.org/INSA-Lyon/1_September_2010 1]
| [http://2010.igem.org/INSA-Lyon/2_September_2010 2]
| [http://2010.igem.org/INSA-Lyon/3_September_2010 3]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/4_September_2010&action=edit 4]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/5_September_2010&action=edit 5]
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[http://2010.igem.org/INSA-Lyon/6_September_2010 6]
| [http://2010.igem.org/INSA-Lyon/7_September_2010 7]
| [http://2010.igem.org/INSA-Lyon/8_September_2010 8]
| [http://2010.igem.org/INSA-Lyon/9_September_2010 9]
| [http://2010.igem.org/INSA-Lyon/10_September_2010 10]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/11_September_2010&action=edit 11]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/12_September_2010&action=edit 12]
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[http://2010.igem.org/INSA-Lyon/13_September_2010 13]
| [http://2010.igem.org/INSA-Lyon/14_September_2010 14]
| [http://2010.igem.org/INSA-Lyon/15_September_2010 15]
| [http://2010.igem.org/INSA-Lyon/16_September_2010 16]
| [http://2010.igem.org/INSA-Lyon/17_September_2010 17]
| [http://2010.igem.org/INSA-Lyon/18_September_2010 18]
| [http://2010.igem.org/INSA-Lyon/19_September_2010 19]
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[http://2010.igem.org/INSA-Lyon/20_September_2010 20]
| [http://2010.igem.org/INSA-Lyon/21_September_2010 21]
| [http://2010.igem.org/INSA-Lyon/22_September_2010 22]
| [http://2010.igem.org/INSA-Lyon/23_September_2010 23]
| [http://2010.igem.org/INSA-Lyon/24_September_2010 24]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/25_September_2010&action=edit 25]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/26_September_2010&action=edit 26]
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[http://2010.igem.org/INSA-Lyon/27_September_2010 27]
| [http://2010.igem.org/INSA-Lyon/28_September_2010 28]
| [http://2010.igem.org/INSA-Lyon/29_September_2010 29]
| [http://2010.igem.org/INSA-Lyon/30_September_2010 30]
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October
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| [http://2010.igem.org/INSA-Lyon/1_October_2010 1]
| [http://2010.igem.org/INSA-Lyon/2_October_2010 2]
| [http://2010.igem.org/INSA-Lyon/3_October_2010 3]
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[http://2010.igem.org/INSA-Lyon/4_October_2010 4]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/5_October_2010&action=edit 5]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/6_October_2010&action=edit 6]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/7_October_2010&action=edit 7]
| [http://2010.igem.org/INSA-Lyon/8_October_2010 8]
| [http://2010.igem.org/INSA-Lyon/9_October_2010 9]
| [http://2010.igem.org/INSA-Lyon/10_October_2010 10]
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[http://2010.igem.org/INSA-Lyon/11_October_2010 11]
| [http://2010.igem.org/INSA-Lyon/12_October_2010 12]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/13_October_2010&action=edit 13]
| [http://2010.igem.org/INSA-Lyon/14_October_2010 14]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/15_October_2010&action=edit 15]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/16_October_2010&action=edit 16]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/17_October_2010&action=edit 17]
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[http://2010.igem.org/wiki/index.php?title=INSA-Lyon/18_October_2010&action=edit 18]
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| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/20_October_2010&action=edit 20]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/21_October_2010&action=edit 21]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/22_October_2010&action=edit 22]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/23_October_2010&action=edit 23]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/24_October_2010&action=edit 24]
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[http://2010.igem.org/wiki/index.php?title=INSA-Lyon/25_October_2010&action=edit 25]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/26_October_2010&action=edit 26]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/27_October_2010&action=edit 27]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/28_October_2010&action=edit 28]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/29_October_2010&action=edit 29]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/30_October_2010&action=edit 30]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/31_October_2010&action=edit 31]
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