INSA-Lyon/23 July 2010

From 2010.igem.org

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<p>DNA Extraction 18A1, 1N1, 14K1, 23L1 (concentrated in 40uL)</p>
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<p>DNA Extraction 18A1, 1N1, 14K1, 23L1, 1F, 24A (concentrated in 40uL)</p>
<p>Agarose gel electrophoresis --> all extractions are OK</p>
<p>Agarose gel electrophoresis --> all extractions are OK</p>
<br>
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     <li>14K:  E+S (30uL)</li>
     <li>14K:  E+S (30uL)</li>
     <li>23L:  E+X (30uL)</li>
     <li>23L:  E+X (30uL)</li>
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    <li>1F: S+P (40uL)</li>
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    <li>24A: X+P (4OuL)</li>
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<p>Agarose gel electrophoresis : no DNA for 23L</p>
<p>Agarose gel electrophoresis : no DNA for 23L</p>
<p>Second digestion of  23L (5uL) with 0,5uL of EcoR1 and 1uL of Xba1 -> gel OK</p>
<p>Second digestion of  23L (5uL) with 0,5uL of EcoR1 and 1uL of Xba1 -> gel OK</p>
 +
<br>
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<p>Extraction and purification of DNA contained on the band 24A with a kit (concentrated in 20uL) </p>
<br>
<br>
<p>Ligations : <br></p>
<p>Ligations : <br></p>
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<li>18A/1N: method 3A (10µL of each and one tube with 2,5µL or 5µL of plasmid C)
<li>18A/1N: method 3A (10µL of each and one tube with 2,5µL or 5µL of plasmid C)
</ol>
</ol>
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<li>1F/24A: ratio 2:1 --> 10/5µL </li>
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Revision as of 13:36, 31 August 2010





July 23

DNA Extraction 18A1, 1N1, 14K1, 23L1, 1F, 24A (concentrated in 40uL)

Agarose gel electrophoresis --> all extractions are OK


Digestions :

  1. 18A : S+E (30uL)
  2. 1N: X+P (34uL)
  3. 14K: E+S (30uL)
  4. 23L: E+X (30uL)
  5. 1F: S+P (40uL)
  6. 24A: X+P (4OuL)

Agarose gel electrophoresis : no DNA for 23L

Second digestion of 23L (5uL) with 0,5uL of EcoR1 and 1uL of Xba1 -> gel OK


Extraction and purification of DNA contained on the band 24A with a kit (concentrated in 20uL)


Ligations :

  1. 14K/23L
    1. ratio 2:1 --> 10/5µL
    2. ratio 4:1 --> 12/3µL
  2. 18A/1N: method 3A (10µL of each and one tube with 2,5µL or 5µL of plasmid C)
  • 1F/24A: ratio 2:1 --> 10/5µL







    • June
    MTWTFSS
      [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/1_June_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/2_June_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/3_June_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/4_June_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/5_June_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/6_June_2010&action=edit 6]
    [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/7_June_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/8_June_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/9_June_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/10_June_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/11_June_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/12_June_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/13_June_2010&action=edit 13]
    [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/14_June_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/15_June_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/16_June_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/17_June_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/18_June_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/19_June_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/20_June_2010&action=edit 20]
    [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/21_June_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/22_June_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/23_June_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/24_June_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/25_June_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/26_June_2010&action=edit 26] [http://2010.igem.org/INSA-Lyon/27_June_2010 27]
    [http://2010.igem.org/INSA-Lyon/28_June_2010 28] [http://2010.igem.org/INSA-Lyon/29_June_2010 29] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/30_June_2010&action=edit 30]
    July
    MTWTFSS
          [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/1_July_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/2_July_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/3_July_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/4_July_2010&action=edit 4]
    [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/5_July_2010&action=edit 5] [http://2010.igem.org/INSA-Lyon/6_July_2010 6] [http://2010.igem.org/INSA-Lyon/7_July_2010 7] [http://2010.igem.org/INSA-Lyon/8_July_2010 8] [http://2010.igem.org/INSA-Lyon/9_July_2010 9] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/10_July_2010&action=edit 10] [http://2010.igem.org/INSA-Lyon/11_July_2010 11]
    [http://2010.igem.org/INSA-Lyon/12_July_2010 12] [http://2010.igem.org/INSA-Lyon/13_July_2010 13] [http://2010.igem.org/INSA-Lyon/14_July_2010 14] [http://2010.igem.org/INSA-Lyon/15_July_2010 15] [http://2010.igem.org/INSA-Lyon/16_July_2010 16] [http://2010.igem.org/INSA-Lyon/17_July_2010 17] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/18_July_2010&action=edit 18]
    [http://2010.igem.org/INSA-Lyon/19_July_2010 19] [http://2010.igem.org/INSA-Lyon/20_July_2010 20] [http://2010.igem.org/INSA-Lyon/21_July_2010 21] [http://2010.igem.org/INSA-Lyon/22_July_2010 22] [http://2010.igem.org/INSA-Lyon/23_July_2010 23] [http://2010.igem.org/INSA-Lyon/24_July_2010 24] [http://2010.igem.org/INSA-Lyon/25_July_2010 25]
    [http://2010.igem.org/INSA-Lyon/26_July_2010 26] [http://2010.igem.org/INSA-Lyon/27_July_2010 27] [http://2010.igem.org/INSA-Lyon/28_July_2010 28] [http://2010.igem.org/INSA-Lyon/29_July_2010 29] [http://2010.igem.org/INSA-Lyon/30_July_2010 30] [http://2010.igem.org/INSA-Lyon/31_July_2010 31]
    August
    MTWTFSS
                [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/1_August_2010&action=edit 1]
    [http://2010.igem.org/INSA-Lyon/2_August_2010 2] [http://2010.igem.org/INSA-Lyon/3_August_2010 3] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/4_August_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/5_August_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/6_August_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/7_August_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/8_August_2010&action=edit 8]
    [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/9_August_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/10_August_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/11_August_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/12_August_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/13_August_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/14_August_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/15_August_2010&action=edit 15]
    [http://2010.igem.org/INSA-Lyon/16_August_2010 16] [http://2010.igem.org/INSA-Lyon/17_August_2010 17] [http://2010.igem.org/INSA-Lyon/18_August_2010 18] [http://2010.igem.org/INSA-Lyon/19_August_2010 19] [http://2010.igem.org/INSA-Lyon/20_August_2010 20] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/21_August_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/22_August_2010&action=edit 22]
    [http://2010.igem.org/INSA-Lyon/23_August_2010 23] [http://2010.igem.org/INSA-Lyon/24_August_2010 24] [http://2010.igem.org/INSA-Lyon/25_August_2010 25] [http://2010.igem.org/INSA-Lyon/26_August_2010 26] [http://2010.igem.org/INSA-Lyon/27_August_2010 27] [http://2010.igem.org/INSA-Lyon/28_August_2010 28] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/29_August_2010&action=edit 29]
    [http://2010.igem.org/INSA-Lyon/30_August_2010 30] [http://2010.igem.org/INSA-Lyon/31_August_2010 31]
    September
    MTWTFSS
        [http://2010.igem.org/INSA-Lyon/1_September_2010 1] [http://2010.igem.org/INSA-Lyon/2_September_2010 2] [http://2010.igem.org/INSA-Lyon/3_September_2010 3] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/4_September_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/5_September_2010&action=edit 5]
    [http://2010.igem.org/INSA-Lyon/6_September_2010 6] [http://2010.igem.org/INSA-Lyon/7_September_2010 7] [http://2010.igem.org/INSA-Lyon/8_September_2010 8] [http://2010.igem.org/INSA-Lyon/9_September_2010 9] [http://2010.igem.org/INSA-Lyon/10_September_2010 10] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/11_September_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/12_September_2010&action=edit 12]
    [http://2010.igem.org/INSA-Lyon/13_September_2010 13] [http://2010.igem.org/INSA-Lyon/14_September_2010 14] [http://2010.igem.org/INSA-Lyon/15_September_2010 15] [http://2010.igem.org/INSA-Lyon/16_September_2010 16] [http://2010.igem.org/INSA-Lyon/17_September_2010 17] [http://2010.igem.org/INSA-Lyon/18_September_2010 18] [http://2010.igem.org/INSA-Lyon/19_September_2010 19]
    [http://2010.igem.org/INSA-Lyon/20_September_2010 20] [http://2010.igem.org/INSA-Lyon/21_September_2010 21] [http://2010.igem.org/INSA-Lyon/22_September_2010 22] [http://2010.igem.org/INSA-Lyon/23_September_2010 23] [http://2010.igem.org/INSA-Lyon/24_September_2010 24] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/25_September_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/26_September_2010&action=edit 26]
    [http://2010.igem.org/INSA-Lyon/27_September_2010 27] [http://2010.igem.org/INSA-Lyon/28_September_2010 28] [http://2010.igem.org/INSA-Lyon/29_September_2010 29] [http://2010.igem.org/INSA-Lyon/30_September_2010 30]
    October
    MTWTFSS
            [http://2010.igem.org/INSA-Lyon/1_October_2010 1] [http://2010.igem.org/INSA-Lyon/2_October_2010 2] [http://2010.igem.org/INSA-Lyon/3_October_2010 3]
    [http://2010.igem.org/INSA-Lyon/4_October_2010 4] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/5_October_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/6_October_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/7_October_2010&action=edit 7] [http://2010.igem.org/INSA-Lyon/8_October_2010 8] [http://2010.igem.org/INSA-Lyon/9_October_2010 9] [http://2010.igem.org/INSA-Lyon/10_October_2010 10]
    [http://2010.igem.org/INSA-Lyon/11_October_2010 11] [http://2010.igem.org/INSA-Lyon/12_October_2010 12] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/13_October_2010&action=edit 13] [http://2010.igem.org/INSA-Lyon/14_October_2010 14] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/15_October_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/16_October_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/17_October_2010&action=edit 17]
    [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/18_October_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/19_October_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/20_October_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/21_October_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/22_October_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/23_October_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/24_October_2010&action=edit 24]
    [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/25_October_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/26_October_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/27_October_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/28_October_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/29_October_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/30_October_2010&action=edit 30] [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/31_October_2010&action=edit 31]

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