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Team:INSA-Lyon/Project/Future direction
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<h3><font color="purple">Uses</font></h3> | <h3><font color="purple">Uses</font></h3> | ||
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| - | <p>We added a very interesting part <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K342002">(phasin-phasin-intein)</a> to the registry which should bind to the PHB granule surface and enable an easier purification way for molecules of interest thanks to the self cleaving protein. | + | <p>We added a very interesting part <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K342002">(phasin-phasin-intein)</a> to the registry which should bind to the PHB granule surface and enable an easier purification way for molecules of interest thanks to the self cleaving protein. Our first goal was to use a Phasin-Phasin-Intein-GFP fusion to test the targeting of that kind of construction to the granule. But there are also a lot of work to adapt this purification way for proteins or molecule of interest can be carried out further.<br> |
Concerning the use of the granule as lipid storage, we didn't manage to co-localize the granules and the lycopene inside the bacteria. We couldn't manage to differentiate the lycopene and PHB by fluorescence microscopy. A granules extraction experiment could be perform after having induce lycopene production and then look for lycopene inside those extractions. So this idea has still to be deepen down. | Concerning the use of the granule as lipid storage, we didn't manage to co-localize the granules and the lycopene inside the bacteria. We couldn't manage to differentiate the lycopene and PHB by fluorescence microscopy. A granules extraction experiment could be perform after having induce lycopene production and then look for lycopene inside those extractions. So this idea has still to be deepen down. | ||
</p> | </p> | ||
Revision as of 22:33, 27 October 2010
Future Direction
Our project was full of ideas and the time too short to achieve until the end our projects. So there is still plenty to do get and improve results.
Production
We managed to produce granules and a new operational PhaC part but we didn't send the complete operon (phaCAB) which would able any team to produce PHB granules inside E.coli.
Uses
We added a very interesting part (phasin-phasin-intein) to the registry which should bind to the PHB granule surface and enable an easier purification way for molecules of interest thanks to the self cleaving protein. Our first goal was to use a Phasin-Phasin-Intein-GFP fusion to test the targeting of that kind of construction to the granule. But there are also a lot of work to adapt this purification way for proteins or molecule of interest can be carried out further.
Concerning the use of the granule as lipid storage, we didn't manage to co-localize the granules and the lycopene inside the bacteria. We couldn't manage to differentiate the lycopene and PHB by fluorescence microscopy. A granules extraction experiment could be perform after having induce lycopene production and then look for lycopene inside those extractions. So this idea has still to be deepen down.
Regulation
We characterize the natural curli promoter inside a plasmid and we wanted to link those results with the ones of our designed curli promoter. But we were not able to realize the measurements to conclude about the performance of our biobrick.
