Team:INSA-Lyon/Protocols/Measurement of fluorescence

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Revision as of 08:10, 25 October 2010







Protocols


  1. Competent cells
  2. Transformation
  3. DNA extraction
  4. Digestion
  5. Ligation
  6. Measure of temperature and shaking speed influence
  7. Measure of osmotic pressure influence
  8. Granules extraction and intein cleavage
  9. Biofilms quantification
  10. Extra






    Measure of fluorescence


    • Introduce 5 mL of half LB in a sterile tube
    • Resuspend bacteria in 0,5 mL of sterile water. Vortex
    • Add 10 µL of the mixture in the LB tube. Vortex
    • Incubate 24 h in a waterbath at the temperature and the shaking speed wished
    • Measure of the fluorescence : input wavelenght 490 nm, output one 510 nm.
    • Dilute 250 µL of culture in 750 µL of water. Measure the biomass (absorbance 600 nm)

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