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Team:HokkaidoU Japan/NotebookTest
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===[[Team:HokkaidoU_Japan/Notebook/August13|Friday, August 13, 2010]]=== | ===[[Team:HokkaidoU_Japan/Notebook/August13|Friday, August 13, 2010]]=== | ||
| - | * PCR amplification of the DNA that we couldn't obtain via miniprep(1-18F) | + | * PCR amplification of the DNA that we couldn't obtain via miniprep previous day(1-18F) |
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Revision as of 15:47, 5 September 2010
since 13 Jul 2010
since 1 Aug 2010
Calendar
| July | ||||||
| SUN | MON | TUE | WED | THU | FRI | SAT |
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | ||||
| 4 | 5 | 6 | 7 | 8 | 9 | 10 |
| 11 | 12 | 13 | 14 | 15 | 16 | 17 |
| 18 | 19 | 20 | 21 | 22 | 23 | 24 |
| 25 | 26 | 27 | 28 | 29 | 30 | 31 |
| August | ||||||
| SUN | MON | TUE | WED | THU | FRI | SAT |
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 |
| 8 | 9 | 10 | 11 | 12 | 13 | 14 |
| 15 | 16 | 17 | 18 | 19 | 20 | 21 |
| 22 | 23 | 24 | 25 | 26 | 27 | 28 |
| 29 | 30 | 31 | ||||
| September | ||||||
| SUN | MON | TUE | WED | THU | FRI | SAT |
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | |||
| 5 | 6 | 7 | 8 | 9 | 10 | 11 |
| 12 | 13 | 14 | 15 | 16 | 17 | 18 |
| 19 | 20 | 21 | 22 | 23 | 24 | 25 |
| 26 | 27 | 28 | 29 | 30 | ||
| October | ||||||
| SUN | MON | TUE | WED | THU | FRI | SAT |
|---|---|---|---|---|---|---|
| 1 | 2 | |||||
| 3 | 4 | 5 | 6 | 7 | 8 | 9 |
| 10 | 11 | 12 | 13 | 14 | 15 | 16 |
| 17 | 18 | 19 | 20 | 21 | 22 | 23 |
| 24 | 25 | 26 | 27 | 28 | 29 | 30 |
| 31 | ||||||
| November | ||||||
| SUN | MON | TUE | WED | THU | FRI | SAT |
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | |
| 7 | 8 | 9 | 10 | 11 | 12 | 13 |
| 14 | 15 | 16 | 17 | 18 | 19 | 20 |
| 21 | 22 | 23 | 24 | 25 | 26 | 27 |
| 28 | 29 | 30 | ||||
Abstract
Monday, July 12, 2010
- The first experiment for us, transformation with BioBrick Devices.
Wednesday, July 21, 2010
- Preparation of competent cells
- Single colony isolation of E. coli which had been transformed on Monday, July 12, 2010
Monday, July 26, 2010
- Cultivation of transformed E. coli for the next day's miniprep
Tuesday, July 27, 2010
- Miniprep
Monday, August 2, 2010
- Restriction enzyme digestion of plasmids which had been purified by miniprep
- Electrophoresis assay
Tuesday, August 3, 2010
Wednesday, August 4, 2010
Thursday, August 5, 2010
Friday, August 6, 2010
Monday, August 9, 2010
- Initial amplification of the BioBrick parts (BBa_I14032 "2-11P", BBa_F2621 "2-21H", BBa_K098995 "3-1E" and BBa_E1010 "1-18F") that are used in the construction of concentration-sensitive and heat-sensitive E. coli
- This is a preliminary experiment before starting the main project
Tuesday, August 10, 2010
- PCR amplification of the other parts (BBa_F1610 "2-24G", BBa_B0034 "1-2M", BBa_B0015 "1-23L" and BBa_K098995 "3-1E") and electrophoresis for confirmation
Wednesday, August 11, 2010
- Preparation for the next day's miniprep
- Estimation of enzyme activity
- Preparation of the glycerol stocks
Thursday, August 12, 2010
- Miniprep (1-18F, 2-21H and 2-11P)
- Electrophoresis of the DNA which had amplified via PCR and miniprep
Friday, August 13, 2010
- PCR amplification of the DNA that we couldn't obtain via miniprep previous day(1-18F)
