INSA-Lyon/11 October 2010
From 2010.igem.org
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<li>phaA/phaB : with E</li> | <li>phaA/phaB : with E</li> | ||
</ol><br></p> | </ol><br></p> | ||
- | <p> | + | <p>Agar gel electrophoresis fro verification and purification : <br> |
<ol id="list_extra"> | <ol id="list_extra"> | ||
<li>phasin and phasin-intein : OK, a lot of DNA, purification</li> | <li>phasin and phasin-intein : OK, a lot of DNA, purification</li> | ||
- | <li>No DNA for all of the other | + | <li>No DNA for all of the other digestions although there is DNA in the extraction</li> |
</ol><br></p> | </ol><br></p> | ||
- | <p> | + | <p>Cultures on plate of phaA/phaB, 12/13, 2P and L2/1N |
<br><br><br><br><br><br><br> | <br><br><br><br><br><br><br> |
Latest revision as of 21:24, 27 October 2010
October 11th
DNA extraction of phasin and phasin-intein
Digestion :
- Phasin-intein, phasin, 2P and L2/1N : with X+P
- Phaisn-intein, phasin, 1N and 18A : with S+P
- L2/1N : with E+P
- phaA/phaB : with E
Agar gel electrophoresis fro verification and purification :
- phasin and phasin-intein : OK, a lot of DNA, purification
- No DNA for all of the other digestions although there is DNA in the extraction
Cultures on plate of phaA/phaB, 12/13, 2P and L2/1N
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