INSA-Lyon/1 October 2010
From 2010.igem.org
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- | <p> | + | <br><p>Blue cells have grown, petri plate stored at room temperature.</p><br> |
+ | <p>PCR PSB1 program on plasmid extraction from 2010/09/23, with new dNTP Roche received on 2010/09/29. Protocol is the same as one from 2010/09/23. Vial 1 corresponded to the negative control with 10µl sterile water ; vial 2 corresponded to the plasmid Amp ; vial 3 corresponded to the plasmid Tet and vial 4 corresponded to the plasmid Cm.</p><br> | ||
+ | <p>Gel verification (0,7%): OK for the sizes of the plasmids Tet et Cm! Control negative is also OK. And PCR on plasmid Amp doesn’t produce a strip of good size (as the last time).</p><br> | ||
+ | <p>Clean-Up on PCR product: vial 3 and 4, and also ompR234 2010/09/20 two PCR products (kit with mini-column MN). 4 elutions are finally stored at -20°C.</p><br> | ||
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Revision as of 12:34, 8 October 2010
Friday, October 1st
Blue cells have grown, petri plate stored at room temperature.
PCR PSB1 program on plasmid extraction from 2010/09/23, with new dNTP Roche received on 2010/09/29. Protocol is the same as one from 2010/09/23. Vial 1 corresponded to the negative control with 10µl sterile water ; vial 2 corresponded to the plasmid Amp ; vial 3 corresponded to the plasmid Tet and vial 4 corresponded to the plasmid Cm.
Gel verification (0,7%): OK for the sizes of the plasmids Tet et Cm! Control negative is also OK. And PCR on plasmid Amp doesn’t produce a strip of good size (as the last time).
Clean-Up on PCR product: vial 3 and 4, and also ompR234 2010/09/20 two PCR products (kit with mini-column MN). 4 elutions are finally stored at -20°C.
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