Team:INSA-Lyon/Protocols/Measurement of fluorescence

From 2010.igem.org

(Difference between revisions)
(New page: <!-- {{INSA-Lyon_style}} --> {{INSA-Lyon/stylegroningen}} {{INSA-Lyon/header}} {{INSA-Lyon/maincontent}} {{INSA-Lyon/menugauche}} <html xmlns="http://www.w3.org/1999/xhtml" dir="ltr" lan...)
 
(2 intermediate revisions not shown)
Line 5: Line 5:
{{INSA-Lyon/menugauche}}
{{INSA-Lyon/menugauche}}
 +
{{INSA-Lyon/menunotebook}}
<html xmlns="http://www.w3.org/1999/xhtml" dir="ltr" lang="en-US" xml:lang="en">
<html xmlns="http://www.w3.org/1999/xhtml" dir="ltr" lang="en-US" xml:lang="en">
Line 30: Line 31:
</script>
</script>
-
<div class="colonnegauche">
 
-
<div id="menug">
 
-
<ul>
 
-
<li><a href="/Team:INSA-Lyon/Project/Theory" class="cteal"> > Theory</a></li>
 
-
<li><a href="/Team:INSA-Lyon/Project/Strategy" class="slateb"> > Strategy</a></li>
 
-
<li><a href="/Team:INSA-Lyon/Project/Results" class="yellow"> > Results</a></li>
 
-
<li><a href="/Team:INSA-Lyon/Project/Future_direction" class="coral"> > Future Direction</a></li>
 
-
<li><a href="/Team:INSA-Lyon/Project/Notebook" class="blue"> > Notebook</a></li>
 
-
<div id="ssmenug">
+
<div id="corps2">
-
<li><a href="/Team:INSA-Lyon/Project/Notebook/Calendar" class="cteal"> > Calendar</a></li>
+
-
<li><a href="/Team:INSA-Lyon/Project/Notebook/Protocols" class="brn"> > Protocols</a></li>
+
-
</div>
+
-
 
+
-
<li><a href="/Team:INSA-Lyon/Project/Modeling" class="green"> > Modeling</a></li>
+
-
<li><a href="/Team:INSA-Lyon/Project/References" class="orange"> > References</a></li>
+
-
+
-
</ul>
+
-
</div>
+
-
</div>
+
-
 
+
-
<div class="colonnedroite">
+
-
<p>
+
-
<br><br>
+
-
<br>
+
-
</p>
+
-
</div>
+
-
 
+
-
<div id="corps">
+
<h3> Protocols </h3>
<h3> Protocols </h3>
 +
<br><p>Choose a protocol to read its description :
<br>
<br>
-
<p></p>
+
<br></p>
</html>
</html>
{{Template:INSALyon2010_Protocole}}
{{Template:INSALyon2010_Protocole}}
Line 68: Line 43:
<html>
<html>
<br><br><br><br><br>
<br><br><br><br><br>
-
<h5 style="text-indent:20px">Measure of fluorescence</h5>
+
<h5 style="text-indent:20px">Measure of temperature and shaking speed influence</h5>
<br><br>
<br><br>
-
<ul>
+
<ul style="margin-left:-65px;">
<li>Introduce 5 mL of half LB in a sterile tube</li>
<li>Introduce 5 mL of half LB in a sterile tube</li>
<li>Resuspend bacteria in 0,5 mL of sterile water. Vortex</li>
<li>Resuspend bacteria in 0,5 mL of sterile water. Vortex</li>
Line 78: Line 53:
<li>Measure of the fluorescence : input wavelenght 490 nm, output one 510 nm.</li>
<li>Measure of the fluorescence : input wavelenght 490 nm, output one 510 nm.</li>
<li>Dilute 250 µL of culture in 750 µL of water. Measure the biomass (absorbance 600 nm)</li>
<li>Dilute 250 µL of culture in 750 µL of water. Measure the biomass (absorbance 600 nm)</li>
-
 
+
<br />
</div>
</div>
</html>
</html>

Latest revision as of 11:53, 26 October 2010




Protocols


Choose a protocol to read its description :

  1. Competent cells
  2. Transformation
  3. DNA extraction
  4. Digestion
  5. Ligation
  6. Measure of temperature and shaking speed influence
  7. Measure of osmotic pressure influence
  8. Granules extraction and intein cleavage
  9. Biofilms quantification
  10. Extra






    Measure of temperature and shaking speed influence


    • Introduce 5 mL of half LB in a sterile tube
    • Resuspend bacteria in 0,5 mL of sterile water. Vortex
    • Add 10 µL of the mixture in the LB tube. Vortex
    • Incubate 24 h in a waterbath at the temperature and the shaking speed wished
    • Measure of the fluorescence : input wavelenght 490 nm, output one 510 nm.
    • Dilute 250 µL of culture in 750 µL of water. Measure the biomass (absorbance 600 nm)

Retrieved from "http://2010.igem.org/Team:INSA-Lyon/Protocols/Measurement_of_fluorescence"