INSA-Lyon/7 September 2010
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Latest revision as of 19:52, 25 October 2010
September 7th
Digestion ( with the Roche enzyme) :
- I0500, 4O : E+S
- 24A, 10L : X+P
Agarose gel electrophoris for the purification of pha A and pha B from the PCR of the 06/09
Digestion (Roche) :
- pha A, pha C : E+S
- pha B : X+P
3 purification of PHL1273 in plate LB+Kan
Liquid cultur of Phasin and Curli and DNA extraction
Second purification of the 2 clones of 18A/24A (clone from the first purification)
Ligation and transformation of :
- 8C/14K in Backbone tet
- 18A/24A in backbone tet
- 4O/10L in backbone tet
- L3/18A in backbone amp
- 12/13 in backbone amp
- pha A/pha B in backbone amp
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