INSA-Lyon/31 August 2010
From 2010.igem.org
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<p>Agarose gel of verification reveal it didn't work</p><br> | <p>Agarose gel of verification reveal it didn't work</p><br> | ||
<p>Extraction of 7P and 22B</p> | <p>Extraction of 7P and 22B</p> | ||
- | <p>Transformation of Curli, Phasin | + | <p>Transformation of Curli, Phasin , 7B, 24M, 22B</p> |
<br><br><br><br><br><br><br> | <br><br><br><br><br><br><br> | ||
Latest revision as of 19:46, 25 October 2010
August 31st
Verification of the PCR :
- pha A : OK
- pha B : wrong temperature of hybridation
New PCR for pha B with a new temperature of hybridation
PCR with universel primers in order to check the ligation 1N/L2
Agarose gel of verification reveal it didn't work
Extraction of 7P and 22B
Transformation of Curli, Phasin , 7B, 24M, 22B
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