Team:KIT-Kyoto/Notebook/Promoters

From 2010.igem.org

(Difference between revisions)
(Data)
(Data)
Line 353: Line 353:
:[[Image:k3.jpg]]
:[[Image:k3.jpg]]
 +
 +
:<span id="a">'''ahpC'''</span>
:<span id="a">'''ahpC'''</span>
 +
<TABLE BORDER="0"><TR>
 +
<TD>n=1</TD></TR>
 +
<TR>
 +
<TD><TABLE BORDER="1"><TR>
 +
<TD ROWSPAN="4">10min</TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1µM</TD><TD>10µM</TD><TD>100µM</TD><TD>1mM</TD></TR>
 +
<TR>
 +
<TD>6.362</TD><TD>6.549</TD><TD>6.639</TD><TD>5.949</TD><TD>7.186</TD><TD>9.847</TD><TD>10.41</TD><TD>12.03</TD></TR>
 +
<TR>
 +
<TD>6.476</TD><TD>6.705</TD><TD>6.733</TD><TD>6.106</TD><TD>7.341</TD><TD>10.22</TD><TD>10.99</TD><TD>12.44</TD></TR>
 +
<TR>
 +
<TD>6.523</TD><TD>6.723</TD><TD>6.779</TD><TD>6.152</TD><TD>7.458</TD><TD>10.4</TD><TD>11.34</TD><TD>12.78</TD></TR>
 +
</TABLE></TD></TR>
 +
<TR>
 +
<TD><TABLE BORDER="1"><TR>
 +
<TD ROWSPAN="3">20min</TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1µM</TD><TD>10µM</TD><TD>100µM</TD><TD>1mM</TD></TR>
 +
<TR>
 +
<TD>7.824</TD><TD>6.91</TD><TD>7.313</TD><TD>6.918</TD><TD>7.257</TD><TD>11.4</TD><TD>12.64</TD><TD>13.56</TD></TR>
 +
<TR>
 +
<TD>7.894</TD><TD>7.043</TD><TD>7.469</TD><TD>6.967</TD><TD>7.373</TD><TD>11.4</TD><TD>12.94</TD><TD>13.86</TD></TR>
 +
</TABLE></TD></TR>
 +
<TR>
 +
<TD><TABLE BORDER="1"><TR>
 +
<TD ROWSPAN="3">30min</TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1µM</TD><TD>10µM</TD><TD>100µM</TD><TD>1mM</TD></TR>
 +
<TR>
 +
<TD>8.171</TD><TD>8.195</TD><TD>8.624</TD><TD>8.713</TD><TD>8.967</TD><TD>11.28</TD><TD>13.64</TD><TD>16.13</TD></TR>
 +
<TR>
 +
<TD>8.32</TD><TD>8.302</TD><TD>8.791</TD><TD>8.83</TD><TD>8.983</TD><TD>11.39</TD><TD>13.94</TD><TD>16.11</TD></TR>
 +
</TABLE></TD></TR>
 +
<TR>
 +
<TD><TABLE BORDER="1"><TR>
 +
<TD ROWSPAN="3">40min</TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1µM</TD><TD>10µM</TD><TD>100µM</TD><TD>1mM</TD></TR>
 +
<TR>
 +
<TD>8.366</TD><TD>8.753</TD><TD>9.2</TD><TD>8.517</TD><TD>8.855</TD><TD>11.49</TD><TD>14.86</TD><TD>15.97</TD></TR>
 +
<TR>
 +
<TD>8.386</TD><TD>8.733</TD><TD>9.19</TD><TD>8.55</TD><TD>9.053</TD><TD>11.87</TD><TD>15.28</TD><TD>16.2</TD></TR>
 +
</TABLE></TD></TR>
 +
<TR>
 +
<TD><TABLE BORDER="1"><TR>
 +
<TD ROWSPAN="3">50min</TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1µM</TD><TD>10µM</TD><TD>100µM</TD><TD>1mM</TD></TR>
 +
<TR>
 +
<TD>10.32</TD><TD>10.47</TD><TD>11.49</TD><TD>11.09</TD><TD>11.93</TD><TD>11.11</TD><TD>16.64</TD><TD>19.77</TD></TR>
 +
<TR>
 +
<TD>10.54</TD><TD>10.79</TD><TD>11.81</TD><TD>11.46</TD><TD>12.32</TD><TD>11.74</TD><TD>17.19</TD><TD>20.18</TD></TR>
 +
</TABLE></TD></TR>
 +
<TR>
 +
<TD><TABLE BORDER="1"><TR>
 +
<TD ROWSPAN="3">60min</TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1µM</TD><TD>10µM</TD><TD>100µM</TD><TD>1mM</TD></TR>
 +
<TR>
 +
<TD>11.86</TD><TD>13.29</TD><TD>13.03</TD><TD>13.3</TD><TD>13.81</TD><TD>16.01</TD><TD>19.44</TD><TD>22.54</TD></TR>
 +
<TR>
 +
<TD>12.08</TD><TD>13.65</TD><TD>13.3</TD><TD>13.45</TD><TD>14.04</TD><TD>16.26</TD><TD>19.82</TD><TD>23.05</TD></TR>
 +
</TABLE></TD></TR>
 +
<TR>
 +
<TD><TABLE BORDER="1"><TR>
 +
<TD ROWSPAN="3">70min</TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1µM</TD><TD>10µM</TD><TD>100µM</TD><TD>1mM</TD></TR>
 +
<TR>
 +
<TD>16.7</TD><TD>16.02</TD><TD>15.77</TD><TD>15.73</TD><TD>18.03</TD><TD>21.17</TD><TD>24.22</TD><TD>24.95</TD></TR>
 +
<TR>
 +
<TD>17.16</TD><TD>16.61</TD><TD>16.37</TD><TD>16.04</TD><TD>18.46</TD><TD>21.51</TD><TD>24.87</TD><TD>25.23</TD></TR>
 +
</TABLE></TD></TR>
 +
<TR>
 +
<TD><TABLE BORDER="1"><TR>
 +
<TD ROWSPAN="4">80min</TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1µM</TD><TD>10µM</TD><TD>100µM</TD><TD>1mM</TD></TR>
 +
<TR>
 +
<TD>21.18</TD><TD>27.04</TD><TD>22.18</TD><TD>23.44</TD><TD>23.54</TD><TD>25.6</TD><TD>28.86</TD><TD>31.32</TD></TR>
 +
<TR>
 +
<TD>22</TD><TD>27.74</TD><TD>22.82</TD><TD>24.1</TD><TD>24.06</TD><TD>25.71</TD><TD>29.14</TD><TD>31.64</TD></TR>
 +
</TABLE></TD></TR>
 +
<TR>
 +
<TD><TABLE BORDER="1"><TR>
 +
<TD>ahpC</TD><TD>H<SUB>2</SUB>O<SUB>2</SUB></TR>
 +
<TR>
 +
<TD></TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1µM</TD><TD>10µM</TD><TD>100µM</TD><TD>1mM</TD></TR>
 +
<TR>
 +
<TD>10min</TD><TD>6.453666667</TD><TD>6.659</TD><TD>6.717</TD><TD>6.069</TD><TD>7.328333333</TD><TD>10.15566667</TD><TD>10.91333333</TD><TD>12.41666667</TD></TR>
 +
<TR>
 +
<TD>20min</TD><TD>7.859</TD><TD>6.9765</TD><TD>7.391</TD><TD>6.9425</TD><TD>7.315</TD><TD>11.4</TD><TD>12.79</TD><TD>13.71</TD></TR>
 +
<TR>
 +
<TD>30min</TD><TD>8.2455</TD><TD>8.2485</TD><TD>8.7075</TD><TD>8.7715</TD><TD>8.975</TD><TD>11.335</TD><TD>13.79</TD><TD>16.12</TD></TR>
 +
<TR>
 +
<TD>40min</TD><TD>8.376</TD><TD>8.743</TD><TD>9.195</TD><TD>8.5335</TD><TD>8.954</TD><TD>11.68</TD><TD>15.07</TD><TD>16.085</TD></TR>
 +
<TR>
 +
<TD>50min</TD><TD>10.43</TD><TD>10.63</TD><TD>11.65</TD><TD>11.275</TD><TD>12.125</TD><TD>11.425</TD><TD>16.915</TD><TD>19.975</TD></TR>
 +
<TR>
 +
<TD>60min</TD><TD>11.97</TD><TD>13.47</TD><TD>13.165</TD><TD>13.375</TD><TD>13.925</TD><TD>16.135</TD><TD>19.63</TD><TD>22.795</TD></TR>
 +
<TR>
 +
<TD>70min</TD><TD>16.93</TD><TD>16.315</TD><TD>16.07</TD><TD>15.885</TD><TD>18.245</TD><TD>21.34</TD><TD>24.545</TD><TD>25.09</TD></TR>
 +
<TR>
 +
<TD>80min</TD><TD>16.93</TD><TD>16.315</TD><TD>16.07</TD><TD>15.885</TD><TD>18.245</TD><TD>21.34</TD><TD>24.545</TD><TD>25.09</TD></TR>
 +
</TABLE></TD></TR></TABLE>
 +
[[Image:a1.jpg]]

Revision as of 21:14, 25 October 2010



Language : English / Japanese


各種プロモーター活性

Contents

K121013
ahpC
sufA
tetR

Data

K121013
n=1
10minless1nM10nM100nM1µM10µM100µM1mM
0.37340.30820.28860.29340.32830.27160.31720.3384
0.37170.31270.2740.28450.32450.26070.29670.3267
20minless1nM10nM100nM1µM10µM100µM1mM
0.34630.32860.32060.2720.36630.28030.47920.3242
0.35340.34330.31970.27540.35580.27990.46120.3265
30minless1nM10nM100nM1µM10µM100µM1mM
0.32740.28930.3310.35640.35190.3530.34430.4742
0.33890.29320.33310.3650.3580.3680.33990.4669
40minless1nM10nM100nM1µM10µM100µM1mM
0.43850.46280.41590.46560.46120.31860.47480.4191
0.45020.46790.40070.4880.44070.31390.45870.4181
50minless1nM10nM100nM1µM10µM100µM1mM
0.3140.44480.35510.45220.34170.35330.44150.3924
0.30910.49110.36980.47070.33830.3480.42380.3919
60minless1nM10nM100nM1µM10µM100µM1mM
0.46790.47670.45920.45910.44610.35440.51540.4195
70minless1nM10nM100nM1µM10µM100µM1mM
0.45210.44790.45340.50950.41390.45640.41580.4354
0.43380.43380.52120.39950.42090.42830.40150.4227
80minless1nM10nM100nM1µM10µM100µM1mM
0.43880.45840.4460.4170.50970.24790.40060.4278
0.42730.43020.42590.41290.47720.24750.40120.4315
K121013H2O2
less1nM10nM100nM1µM10µM100µM1mM
10min0.372550.310450.28130.288950.32640.266150.306950.33255
20min0.349850.335950.320150.27370.361050.28010.47020.32535
30min0.333150.291250.332050.36070.354950.36050.34210.47055
40min0.444350.465350.40830.47680.450950.316250.466750.4186
50min0.311550.467950.362450.461450.340.350650.432650.39215
60min0.46790.47670.45920.45910.44610.35440.51540.4195
70min0.442950.440850.48730.45450.41740.442350.408650.42905
80min0.433050.44430.435950.414950.493450.24770.40090.42965
K1.jpg
n=2
10minless1nM10nM100nM1µM10µM100µM1mM
0.32390.28240.3270.29980.32920.30090.30640.3619
0.32380.2860.31480.29930.32030.30570.30640.3672
0.32050.29380.31250.29760.3270.31020.31490.3667
20minless1nM10nM100nM1µM10µM100µM1mM
0.46350.45750.43640.40511.2390.43950.380.4392
0.43840.43180.40030.3761.2240.42490.35040.4516
0.41880.42130.39890.37281.2320.41280.37380.4561
30minless1nM10nM100nM1µM10µM100µM1mM
0.34590.35340.44060.3330.42580.38520.36410.4019
0.35280.34540.43160.34030.40870.3720.3680.4156
0.34630.34630.41610.34210.40050.36550.35950.405
40minless1nM10nM100nM1µM10µM100µM1mM
0.3990.46120.41310.54110.41630.39520.50980.3716
0.36970.46140.39640.52410.40310.37680.50630.3774
0.36780.44960.39720.5430.39570.37720.47950.379
50minless1nM10nM100nM1µM10µM100µM1mM
0.50010.42910.35490.44070.56560.46180.44960.4137
0.46940.40710.34090.40450.56430.42140.41790.4079
0.43420.38990.33890.38380.57480.40580.40870.4129
60minless1nM10nM100nM1µM10µM100µM1mM
0.40460.39840.39510.36510.44160.41420.40310.4112
0.38840.40480.3860.36490.41490.40630.41320.4091
0.40260.40420.38890.36950.40450.41360.41730.4046
70minless1nM10nM100nM1µM10µM100µM1mM
0.42620.59950.55670.41270.45360.44960.4340.3871
0.4260.54480.50360.42120.45370.46170.42870.3929
0.43580.50570.49950.43050.4650.46630.44640.404
80minless1nM10nM100nM1µM10µM100µM1mM
0.45730.44530.45630.45720.46390.46620.45010.4169
0.47430.45820.46770.47120.47720.48360.46510.43
0.4740.46020.470.48470.48070.48970.46820.4156
90minless1nM10nM100nM1µM10µM100µM1mM
0.46920.45750.49070.47480.48330.44420.53290.4461
0.48180.46380.44960.48110.49310.44310.54260.4504
0.4910.46670.50770.48550.50630.4520.53990.4476
K121013H2O2
less1nM10nM100nM1µM10µM100µM1mM
10min0.3227333330.28740.31810.29890.32550.30560.3092333330.365266667
20min0.4402333330.4368666670.4118666670.3846333331.2316666670.4257333330.3680666670.448966667
30min0.3483333330.3483666670.4294333330.3384666670.4116666670.3742333330.3638666670.4075
40min0.46790.40870.34490.4096666670.5682333330.4296666670.42540.4115
50min0.3985333330.4024666670.390.36650.4203333330.4113666670.41120.4083
60min0.4293333330.550.5199333330.4214666670.4574333330.45920.4363666670.394666667
70min0.4685333330.4545666670.4646666670.4710333330.4739333330.4798333330.4611333330.420833333
80min0.4806666670.4626666670.4826666670.4804666670.4942333330.4464333330.5384666670.448033333
K2.jpg
n=3
10minless1nM10nM100nM1µM10µM100µM1mM
0.67360.66150.68380.66310.72250.64560.66740.6824
0.67470.66660.67850.66680.72330.65510.65930.6977
0.67930.66170.68220.66060.73110.66080.64740.6928
20minless1nM10nM100nM1µM10µM100µM1mM
0.71460.70380.71160.7050.69540.69610.76761.056
0.71520.68960.69820.70560.6940.69540.75321.038
0.71370.70810.70470.69910.70910.70120.73591.043
30minless1nM10nM100nM1µM10µM100µM1mM
0.80890.76980.66440.70440.75870.68590.84380.7259
0.79190.75960.65870.71520.75870.69230.80640.7172
0.81090.77460.68140.71090.75820.69930.81630.7161
40minless1nM10nM100nM1µM10µM100µM1mM
0.76810.72850.71270.7040.79950.78970.7760.7421
0.76590.72620.70250.70970.79640.78780.76850.7498
0.77210.73070.70680.71680.80210.78510.77280.7586
50minless1nM10nM100nM1µM10µM100µM1mM
0.72190.72920.75540.78370.8140.84180.83840.9178
0.73320.73230.75920.78570.820.84760.84310.9303
0.74010.73480.76660.79290.82490.84580.8380.9356
60minless1nM10nM100nM1µM10µM100µM1mM
0.90140.88450.98140.86610.83370.88810.8440.9678
0.9080.89680.98810.87330.81890.89460.83480.9633
0.91440.89820.98510.88660.84310.88090.83330.956
70minless1nM10nM100nM1µM10µM100µM1mM
0.9420.98450.96670.91660.91350.90990.97040.9324
0.94110.97210.9550.89980.91070.89650.94030.931
0.94220.96630.95670.89970.90320.87890.95280.9409
80minless1nM10nM100nM1µM10µM100µM1mM
1.0381.0130.99441.0241.0251.0120.99511.167
1.0181.010.98681.0080.96910.99530.97381.14
1.0341.0241.0111.0010.97610.9990.97941.144
90minless1nM10nM100nM1µM10µM100µM1mM
0.97911.0111.0531.0231.0370.98860.93251.116
0.99711.0061.0631.0130.99481.0070.95131.108
0.98181.0111.0711.0381.0231.0020.94921.098
K121013H2O2
less1nM10nM100nM1µM10µM100µM1mM
10min0.6758666670.6632666670.68150.66350.7256333330.6538333330.6580333330.690966667
20min0.71450.70050.7048333330.7032333330.69950.6975666670.7522333331.045666667
30min0.80390.7680.6681666670.7101666670.7585333330.69250.8221666670.719733333
40min0.76870.7284666670.7073333330.7101666670.7993333330.7875333330.7724333330.750166667
50min0.7317333330.73210.76040.7874333330.8196333330.8450666670.8398333330.9279
60min0.9417666670.97430.9594666670.9053666670.9091333330.89510.95450.934766667
70min1.031.0156666670.99741.0110.9900666671.00210.9827666671.150333333
80min0.9861.0093333331.0623333331.0246666671.0182666670.99920.9443333331.107333333
90min0.9861.0093333331.0623333331.0246666671.0182666670.99920.9443333331.107333333
K3.jpg


ahpC
n=1
10minless1nM10nM100nM1µM10µM100µM1mM
6.3626.5496.6395.9497.1869.84710.4112.03
6.4766.7056.7336.1067.34110.2210.9912.44
6.5236.7236.7796.1527.45810.411.3412.78
20minless1nM10nM100nM1µM10µM100µM1mM
7.8246.917.3136.9187.25711.412.6413.56
7.8947.0437.4696.9677.37311.412.9413.86
30minless1nM10nM100nM1µM10µM100µM1mM
8.1718.1958.6248.7138.96711.2813.6416.13
8.328.3028.7918.838.98311.3913.9416.11
40minless1nM10nM100nM1µM10µM100µM1mM
8.3668.7539.28.5178.85511.4914.8615.97
8.3868.7339.198.559.05311.8715.2816.2
50minless1nM10nM100nM1µM10µM100µM1mM
10.3210.4711.4911.0911.9311.1116.6419.77
10.5410.7911.8111.4612.3211.7417.1920.18
60minless1nM10nM100nM1µM10µM100µM1mM
11.8613.2913.0313.313.8116.0119.4422.54
12.0813.6513.313.4514.0416.2619.8223.05
70minless1nM10nM100nM1µM10µM100µM1mM
16.716.0215.7715.7318.0321.1724.2224.95
17.1616.6116.3716.0418.4621.5124.8725.23
80minless1nM10nM100nM1µM10µM100µM1mM
21.1827.0422.1823.4423.5425.628.8631.32
2227.7422.8224.124.0625.7129.1431.64
ahpCH2O2
less1nM10nM100nM1µM10µM100µM1mM
10min6.4536666676.6596.7176.0697.32833333310.1556666710.9133333312.41666667
20min7.8596.97657.3916.94257.31511.412.7913.71
30min8.24558.24858.70758.77158.97511.33513.7916.12
40min8.3768.7439.1958.53358.95411.6815.0716.085
50min10.4310.6311.6511.27512.12511.42516.91519.975
60min11.9713.4713.16513.37513.92516.13519.6322.795
70min16.9316.31516.0715.88518.24521.3424.54525.09
80min16.9316.31516.0715.88518.24521.3424.54525.09

A1.jpg




sufA




tetR