Team:Newcastle/Protocol list

From 2010.igem.org

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This page contains the protocols used by Team Newcastle 2010
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{{Team:Newcastle/mainbanner}}
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*PCR
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==Our Protocol List==
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**[[TeamNewcastleColony PCR|Colony PCR]]
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**Gibson Cloning
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This page contains the many protocols used by Team Newcastle 2010!
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*Transformation of ''E. coli''
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*[[TeamNewcastleNanoDrop Spectrophotometer|Nanodrop Spectrophotometer]]  
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[[Image:Newcastle Protocol.jpeg|380px|right]]
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*Growing a liquid culture  
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*Minipreps
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*[[Team:Newcastle/PCR| PCR]]
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*[[TeamNewcastleLigation|Ligation]]  
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*[[Team:Newcastle/PCR_purification| PCR purification]]
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*[[TeamNewcastleRestriction digests|Restriction digests]]
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*[[Team:Newcastle/DNA_hydration|DNA Hydration]]
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*Gel electrophoresis  
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*[[Team:Newcastle/Gibson Cloning|Gibson cloning]]
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*[[Team:Newcastle/E. coli Competence|Preparing competent ''E. coli'']]
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*Transformation of ''B. subtilis''
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*[[Team:Newcastle/Transformation of E. coli|Transformation of ''E. coli'']]
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*[[Team:Newcastle/Growing an overnight cultures| Growing an overnight culture]]
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*[[Team:Newcastle/Qiagen Minipreps|Qiagen Minipreps]]
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*[[Team:Newcastle/Ligation|Ligation]]  
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*[[Team:Newcastle/Restriction digests| Restriction digests]]
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*[[Team:Newcastle/Gel electrophoresis| Gel electrophoresis]]
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*[[Team:Newcastle/Gel extraction| Gel extraction]]
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*[[TeamNewcastleNanoDrop Spectrophotometer| Nanodrop Spectrophotometer]]
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*[[Team:Newcastle/Transformation of B. subtilis| Transformation of ''Bacillus subtilis'']]
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*[[Team:Newcastle/DNA extraction|DNA extraction of ''Bacillus subtilis'']]
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*[[Team:Newcastle/Arginine test| Arginine test]]
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*[[Team:Newcastle/Urease test| Urease test]]
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*[[Team:Newcastle/Agar plates| Agar plates]]
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*[[Team:Newcastle/Glycerol stocks|Making glycerol stocks]]
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*[[Team:Newcastle/Slide Preparation|Slide Preparation]]
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*[[Team:Newcastle/Extreme Base Resistance Test|Extreme Base Resistance Test]]
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*[[Team:Newcastle/IPTG INduction|Slide preparation for IPTG induced cells]]
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*[[Team:Newcastle/Scanning_EM|Scanning Electron Microscopy]]
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'''Go back to our main [[Team:Newcastle/notebook| Lab book]] page'''
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{{Team:Newcastle/footer}}

Latest revision as of 22:03, 27 October 2010