NanoDrop Spectrophotometer

Nanodrop uses absorbance to measure the concentration and purity of DNA, RNA and protein. The ideal concentration of DNA is 150 ng/ml.

Materials required

• Pipettes
• Nanodrop machine
• Appropriate blanking solution
• Appropriate samples

Procedures

1. Log onto computer and select Nanodrop program from the desktop (ND 1000)
2. Wipe the pedestal and top of the Nanodrop machine with a tissue. Place 3 µl of water to nib of pedestal and press blank to clean.
3. After blanking, wipe the water off and equalize the Nanodrop using 3 μl of the appropriate buffer used to resuspend the sample. (example: Miniprep samples should be equalized with EB buffer).
4. Use DNA-50 for DNA samples.
5. Wipe to remove buffer and apply 3 μl of sample to nib. Press measure.
6. If dealing with multiple samples, clean the equipment with water at regular intervals (about every 10 samples)
7. After measurements, clean the equipment with 3 μl of water on the spectrometer and press blank. Wipe and log off.

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