Making glycerol stocks

Materials

• 1.5ml microcentrifuge tubes
• Glycerol

Protocol

1. Inoculate 5ml THY Broth with a single colony and incubate, stationary at 37°C for 8 hours.
2. 0.5ml culture mixed with 0.5ml of sterile 50% glycerol (filter sterlise).
3. Store at -80°C in microcentrifuge tubes.

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