Team:Newcastle/26 August 2010

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(Difference between revisions)
(Gel extraction)
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===Results===
===Results===
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When we look at the gel through the GelDoc, only pSB1C3 was successfully digested with EcoR1 and Pst1 while ''yneA'' did not show any bands.
+
The gel did not show anything when we looked at it from GelDoc.
===Conclusion===
===Conclusion===
-
We will repeat the protocol again on [[Team:Newcastle/31_August_2010|31.08.10]].
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The restriction digest did not work, so we will repeat the protocol again [[Team:Newcastle/27_August_2010|tomorrow]].

Revision as of 11:18, 31 August 2010

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Contents

yneA

PCR (Repeat)

Aim

To repeat the PCR that we did yesterday using the correct rocF primers.

Materials and Protocol

Please refer to PCR.


PCR Purification

Aim

To remove unwanted primers, taq polymerase, buffer and salts to obtain pure DNA.

Materials and Protocol

Please refer to PCR purification.


Digestion

Aim

To digest the PCR products of pSB1C3 and yneA from PCR purification.

Materials and Protocol

Please refer to restriction digest.


Gel extraction

Aim

To purify the DNA of yneA and pSB1C3 by extracting the bands from the gel after running gel electrophoresis. Concentration of DNA is then checked with NanoDrop.

Materials and Protocol

Please refer to:

Results

The gel did not show anything when we looked at it from GelDoc.

Conclusion

The restriction digest did not work, so we will repeat the protocol again tomorrow.



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