Team:Newcastle/26 August 2010


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Screening Bacillius subtilis transformants


The aim of the experiment is to identify those colones that have the plasmid integrated at the correct position in the chromosome, which is the amyE locus. Those that have integrated at the correct position will not be able to break down starch, which can be tested by exposing the colonies on the starch plates to iodine.


Some of our colonies did not have halos, therefore the transformation and integration was successful for these colonies.

Below you can see cells from these positive colonies under the microscope.

Filamentous cells
Filamentous cells showing GFP signal
Normal Bacillus subtilis 168

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