Team:Freiburg Bioware
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Revision as of 18:00, 12 October 2010
Gene delivery using viral vectors holds great promise for the treatment of acquired and inherited diseases. The human Adeno-Associated Virus (AAV) is a small, non-pathogenic, single-stranded DNA virus gaining increasing attention being both versatile and effective. Taking current knowledge into account, we generated a recombinant, modularized, BioBrick-compatible AAV ‘Virus Construction Kit’. We provide parts for modified capsid proteins, targeting modules, tumor-specific promoters, and prodrug-activating enzymes as well as readily assembled vectors for gene delivery and production of non-replicative virus particles. The viral tropism is altered by N-terminal fusion or by loop replacement of the capsid proteins. Functionality of viruses constructed from our kit was demonstrated by fluorescent protein expression in infected cells and by prodrug-induced killing of tumor cells upon viral delivery of a thymidine kinase. Incorporating multiple layers of safety, we provide a general tool to the growing field of personalized medicine and demonstrate its use in tumor therapy.
Project Results
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This Site is in Construction, later you will find more right here...[more]
Heading:
This Site is in Construction, later you will find more right here...[Klick for more]
Head-Line:
This Site is in Construction, later you will find more right here...[Klick for more]
Virus Construction Kit
Heading:
This site is under construction, later you will find more right here...[more]
Heading:
This site is under construction, later you will find more right here...[Klick for more]
Head-Line:
This site is under construction, later you will find more right here...[Klick for more]
BioBricks
Modularisation and Modification of the viral tropismn and the effector function resulted in many different BioBricks.
Click here to explore them.
Notebook
Head-Line:
This Site is in Construction, later you will find more right here...[Klick for more]