Team:Freiburg Bioware/BioBricks/vector plasmid


BBa_K404100[AAV2]-left-ITRFreiburg Bioware 2010147
BBa_K404101[AAV2]-right-ITR Freiburg Bioware 2010138
BBa_K404108hGH terminatorFreiburg Bioware 2010481
BBa_K404114[AAV2]-left-ITR_pCMV Freiburg Bioware 2010809
BBa_K404115[AAV2]-left-ITR_phTERT Freiburg Bioware 2010612
BBa_K404116hGH_[AAV2]-right-ITR Freiburg Bioware 2010632
BBa_K404117[AAV2]-left-ITR_pCMV_betaglobin Freiburg Bioware 20101310
BBa_K404118[AAV2]-left-ITR_phTERT_betaglobin Freiburg Bioware 20101113
BBa_K404119[AAV2]-left-ITR_pCMV_betaglobin_mVenus_hGH_[AAV2]-right-ITR Freiburg Bioware 20102687
BBa_K404120[AAV2]-left-ITR_pCMV_betaglobin_CFP_hGH_[AAV2]-right-ITR Freiburg Bioware 20102687
BBa_K404121[AAV2]-left-ITR_pCMV_betaglobin_mCherry_hGH_[AAV2]-right-ITR Freiburg Bioware 20102826
BBa_K404122[AAV2]-left-ITR_pCMV_betaglobin_mGMK_TK30_hGH_[AAV2]-right-ITR Freiburg Bioware 20103659
BBa_K404123[AAV2]-left-ITR_phTERT_betaglobin_mVenus_hGH_[AAV2]-right-ITR Freiburg Bioware 20102490
BBa_K404124[AAV2]-left-ITR_phTERT_betaglobin_mGMK_TK30_hGH_[AAV2]-right-ITR Freiburg Bioware 20103462
BBa_K404125[AAV2]-left-ITR_phTERT_betaglobin_CD_hGH_[AAV2]-right-ITR Freiburg Bioware 20103057
BBa_K404126[AAV2]-left-ITR_pCMV_betaglobin_CD_hGH_[AAV2]-right-ITR Freiburg Bioware 20103254
BBa_K404127[AAV2]-left-ITR_pCMV_betaglobin_mVenus_[AAV2]-right-ITR Freiburg Bioware 20102200
BBa_K404128[AAV2]-left-ITR_pCMV_mVenus_hGH_[AAV2]-right-ITR Freiburg Bioware 20102190
BBa_K404129[AAV2]-left-ITR_phTERT_betaglobin_mCherry_hGH_[AAV2]-right-ITR Freiburg Bioware 20102629

Vectorplasmid BioBricks

Vectorplasmid BioBricks allow it to reconstitute a vectorplasmid of your choice. Either the provided parts are assembled or an other Gene of Interest (GOI) can be integrated into an vectorplasmid. Beside the packaging capacity of 4.7 kbb, the possiblilities are virtually endless.
The Inverted Terminal Repeats (ITRs) are constructs form highly stable secondary structures that are recognized by the Rep proteins and function as a packiging signal. There for the ITRs have to flank the later vectorplasmid.
The Beta-globin and the human growth hormon polyadenylation sequence (hGH)are regulatory sequences that are ment to enhace the expression of the used GOI.

Create your own Vectorplasmid with the PrecursorBricks

For several applications users of the Virus Construction Kit will only want to insert their own GOI and not change the promoter and regulatory elements in a first experiment. For these applications we provide precursors of our vectorplasmid that reduce the required cloning on two steps.

Assembled Vectorplasmid - ready for transfection

For the development of retargeted viral vectors the vectorplasmid often functions as generator for different read-outs that can be measured in several assays. For this purpose it is often desired to use fully assembled vectorplasmids that are confirmed working and provieded in our Virus Construction Kit.
There for we assembled vectorplasmids with either fluorescent proteins like mCerulean(CFP), mVenus(YFP) and mCherry or produrg convertases like the cytosine deaminase or the tymindine kinase.
Detection of productive infections is possible by measuring the fluorescence of the fluorescent proteins or the number of killed cells where as the detection of the vectorplasmid is in any step of the infection possible by performing a qPCR reaction with primers for the CMV promoter.