Team:Caltech/Week 15

From 2010.igem.org

(Difference between revisions)
 
Line 20: Line 20:
==Friday 9/24==
==Friday 9/24==
 +
 +
*Sequencing apparently showed that L36 and L37 did not ligate properly at the second stage of PCR ligation.
 
 
}}
}}

Latest revision as of 18:16, 17 October 2010


iGEM 2010



Home

People

Project Details

Protocols

Completed Systems

Notebook

Biosafety

Human Impact

References

Support


Caltech logo watermark.png


Monday 9/20

  • Transformation of completed sigma32 (HtpR) RFC10 BioBrick construction into electrocompetent DH5 alpha cells

Tuesday 9/21

  • Miniprep of DNA from Monday's transformed cells

Wednesday 9/22

  • Verify that miniprepped plasmid DNA is of sufficiently high concentration to send out for sequencing
  • Send out plasmid DNA for sequencing

Thursday 9/23

Friday 9/24

  • Sequencing apparently showed that L36 and L37 did not ligate properly at the second stage of PCR ligation.


 
Error creating thumbnail: /websites/2010.igem.org/wiki/bin/ulimit4.sh: line 4: convert: command not found
Caltech footer.png


Locations of visitors to this page