Monday 9/6
- Colony PCR cultures of L95 (HSP-LacI-EYFP) cells imaged under microscope
- Cells are vastly overgrown and unhealthy (cultures visualized after one weekend)
- Fresh cultures of L95 cells grown in preparation for a proper microscope imaging session
Tuesday 9/7
- Digested successful sigma32 (HtpR) BioBrick in preparation for ligation into pSB1C3 chloramphenicol backbone
- Treat the digestion like a backbone digestion
- Ligated the digest into pSB1C3
- Cleaned up the lab in preparation for mandatory leave of absence
Wednesday 9/8
- Photographed the results of transglutaminase long-term testing (6 week span)
- Results are stunning: TGase + cells, TGase + cell lysate forms a dark grey-colored brick about 7mm diameter (culture tube was approximately 13-14mm inside diameter!)
- Several TGase + acid and TGase + base show very dark, vivid colors unattributable to the acid/base
- Mandatory leave of absence begins
Thursday 9/9
The lab is being used by a class.
Friday 9/10
The lab is being used by a class.
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