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Monday 9/20
- Transformation of completed sigma32 (HtpR) RFC10 BioBrick construction into electrocompetent DH5 alpha cells
Tuesday 9/21
- Miniprep of DNA from Monday's transformed cells
Wednesday 9/22
- Verify that miniprepped plasmid DNA is of sufficiently high concentration to send out for sequencing
- Send out plasmid DNA for sequencing
Thursday 9/23
Friday 9/24
- Sequencing apparently showed that L36 and L37 did not ligate properly at the second stage of PCR ligation.
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