Team:Caltech/Week 1

Monday 6/14

• Discussed procuring items & lab space.
• Prepared for SURF start.

Tuesday 6/15

• Created GANTT Chart (right) detailing our work plan for the summer.

GANTT Chart diagramming our planned progress throughout the summer.

• Detailed possible side projects, open to any member as time permits:
  • Agarase
  • pH/Environmental Response
  • Phototaxis
  • Colored plastic
  • Activa (transglutaminase)
• Procured supplies from stock room
• Cleaned out lab space
• Prepared LB-amp media & plates

Wednesday 6/16

• Procured remaining antibiotics & sample of XL1 Blue cells
• Prepare orders for Sigma, NEB, and others
  • Sigma: Crosslinking compounds, substrates (linseed oil & soybean oil)
  • NEB: Biobrick assembly kit, Ladders, polymerase
• Prepared list of biobricks to order
  • Addl Lysis bricks
  • Agarase bricks

Thursday 6/17

• Made stock solutions for transformation and subsequent culturing
• Transformed XL-1 Blue E. Coli with selected BioBricks
  • [http://partsregistry.org/wiki/index.php?title=Part:BBa_J09250 J09250], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K124017 K124017], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K124014 K124014], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 B0015], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13601 I13601], pBAD 18 plasmid
• Ordered more BioBricks:
  • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K112012 K112012]
  • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K108001 K108001]
  • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K112806 K112806]
  • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K284001 K284001]
  • [http://partsregistry.org/wiki/index.php?title=Part:BBa_I737020 I737020]

(We also wanted to order sequencing primers [http://partsregistry.org/wiki/index.php?title=Part:BBa_G00100 G00100] & [http://partsregistry.org/wiki/index.php?title=Part:BBa_G00101 G00101], but apparently those aren't sent to teams.)

Friday 6/18

• Checked transformation
• Minipreped transformed bacteria
  • Checked the plasmid concentration in the mini-prep solution
• Made glycerol stocks from the transformed plasmid