Team:KIT-Kyoto/Protocol/English

Plasmid Mini Prep

• Buffer P1 (Suspension Buffer)

50mM Tris-HCl and 10mM EDTA, pH8.0(25℃)50μg/ml

• Buffer P2 (Lysis Buffer)

0.2M NaOH and 1%SDS

• Buffer N3 (Neutralization and Binding Buffer)

4M guanidine hydrochloride and 0.5M potassium acetate, pH4.2

• Buffer PB (Wash Buffer)

5M guanidine hydrochloride,20mM Tris-HCl, pH6.6 (25℃) [final concentrations after addition of ethanol]with 38% ethanol.

• Buffer PE (Wash Buffer)

20mM NaCl, 2mM Tris-HCl, pH7.5 (25℃) [final concentrations after addition of ethanol:widh 80% ethanol

• Buffer EB(Elution Buffer)

10mM Tris-HCl, pH8.5(25℃)