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Team:Newcastle/26 August 2010
From 2010.igem.org
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yneA
PCR (Repeat)
Aim
To repeat the PCR that we did yesterday using the correct rocF primers.
Materials and Protocol
Please refer to PCR.
PCR Purification
Aim
To remove unwanted primers, taq polymerase, buffer and salts to obtain pure DNA.
Materials and Protocol
Please refer to PCR purification.
Restriction Digest
Aim
To digest the PCR products of pSB1C3 and yneA from PCR purification with enzymes EcoR1 and Nhe1.
Materials and Protocol
Please refer to restriction digest.
Results, Discussion and Conclusion
We run the digested products with gel electrophoresis to determine whether the digest worked.
Gel extraction
Aim
To purify the DNA of yneA and pSB1C3 by extracting the bands from the gel after running gel electrophoresis. Concentration of DNA is then checked with NanoDrop.
Materials and Protocol
Please refer to:
Results
The gel did not show any band when we looked at it from GelDoc.
Conclusion
The restriction digest did not work, so we will repeat the protocol again tomorrow.
   
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