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Contents 1 yneA 1.1 Aims 1.2 Materials and Protocol 1.3 Results 1.3.1 Nanodrop results 1.3.2 Gel 2 Gel extraction of pSB1C3 plasmid 2.1 Aim 2.2 Materials and Protocol 2.3 Results

yneA

Aims

Today we aim to extract yneAin the biobrick comapatible vector from yesterday's overnight culture. We aim to check the concentration from the miniprep with the nanodrop then digest the plasmid with EcoR1 and Nhe1, ligate into GFPrrnb and transform cells to grow on a plate overnight.

Materials and Protocol

Please refer to protocols mentioned below for materials required:

• Plasmid extraction protocol
• Nanodrop protocol
• Restriction digest protocol
• Gel electrophoresis protocol
• Gel extraction protocol

Results

Nanodrop results

	yneA 1	yneA 2	yneA 3	yneA 4
Concentration of DNA ng/µl	247.3	233.6	456.6

Gel

Gel extraction of pSB1C3 plasmid

Aim

The aim of this experiment is to extract EcoR1 digested pSB1C3 plasmid that have been extracted on 3 August 2010. The digested plasmid is then used for PCR with a new set of primers that have been designed to amplify the pSB1C3 sequence.

Materials and Protocol

Please refer to: Gel electrophoresis and Gel extraction.

Results