Team:Caltech/Week 6

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Monday 7/19

Group meeting!
- Discussed issues with cloning and next week's focus.
Performed a digest (now for 2hr) and ligations (L18-23). Transformed into DH5α.
Ran gel of digest product to verify correct bands.

Digest test gel with US and DS digested bricks. (7/19)

Tuesday 7/20

All ligation transformations failed. Will try 16hr ligations and purifying digest products with a PCR purification kit.
Made new growth curves testing AIBN effect on cells in LB
Performed another live/dead assay
- Had solutions of cells in glycerol and AIBN
- Plated cells every hour for three hours
- Combined glycerol solutions with LB and plated the solution

Wednesday 7/21

Thursday 7/22

Friday 7/23

Weekend 7/24-25

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