Team:Caltech/Week 1

From 2010.igem.org

Revision as of 22:56, 16 July 2010 by Sashabu (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


iGEM 2010



Home

People

Project Details

Protocols

Completed Systems

Notebook

Biosafety

Human Impact

References

Support


Caltech logo watermark.png


Monday 6/14

  • Discussed procuring items & lab space.
  • Prepared for SURF start.

Tuesday 6/15

  • Created GANTT Chart (right) detailing our work plan for the summer.
GANTT Chart diagramming our planned progress throughout the summer.
  • Detailed possible side projects, open to any member as time permits:
    • Agarase
    • pH/Environmental Response
    • Phototaxis
    • Colored plastic
    • Activa (transglutaminase)
  • Procured supplies from stock room
  • Cleaned out lab space
  • Prepared LB-amp media & plates

Wednesday 6/16

  • Procured remaining antibiotics & sample of XL1 Blue cells
  • Prepare orders for Sigma, NEB, and others
    • Sigma: Crosslinking compounds, substrates (linseed oil & soybean oil)
    • NEB: Biobrick assembly kit, Ladders, polymerase
  • Prepared list of biobricks to order
    • Addl Lysis bricks
    • Agarase bricks

Thursday 6/17

  • Made stock solutions for transformation and subsequent culturing
  • Transformed XL-1 Blue E. Coli with selected BioBricks
    • [http://partsregistry.org/wiki/index.php?title=Part:BBa_J09250 J09250], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K124017 K124017], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K124014 K124014], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 B0015], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13601 I13601], pBAD 18 plasmid
  • Ordered more BioBricks:
    • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K112012 K112012]
    • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K108001 K108001]
    • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K112806 K112806]
    • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K284001 K284001]
    • [http://partsregistry.org/wiki/index.php?title=Part:BBa_I737020 I737020]

(We also wanted to order sequencing primers [http://partsregistry.org/wiki/index.php?title=Part:BBa_G00100 G00100] & [http://partsregistry.org/wiki/index.php?title=Part:BBa_G00101 G00101], but apparently those aren't sent to teams.)

Friday 6/18

  • Checked transformation
  • Minipreped transformed bacteria
    • Checked the plasmid concentration in the mini-prep solution
  • Made glycerol stocks from the transformed plasmid
Error creating thumbnail: /websites/2010.igem.org/wiki/bin/ulimit4.sh: line 4: convert: command not found
Caltech footer.png


Locations of visitors to this page

Retrieved from "http://2010.igem.org/Team:Caltech/Week_1"