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Team:KIT-Kyoto/Notebook/Promoters
From 2010.igem.org
Revision as of 07:31, 27 October 2010 by
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Abstract
Introduction
Materials & Methods
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Protocol
Promoters
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Home
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Notebook
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Promoters
First
Week1 (Aug 11-14)
Week2 (Aug 15-21)
Week3 (Aug 22-28)
Week4 (Aug 29-Sep 4)
Week5 (Sep 5-11)
Week6 (Sep 12-18)
Week7 (Sep 19-25)
Week8 (Sep 26-Oct 2)
Week9 (Oct 3-9)
Week10 (Oct 10-13)
First
Week1 (Aug 22-28)
Week2 (Aug 29-Sep4)
Week3 (Sep5-11)
Week4 (Sep12-18)
Week5 (Sep19-25)
Week6 (Sep26-Oct2)
Week7 (Oct3-9)
Week8 (Oct10-16)
Week9 (Oct17-23)
Promoters
>>return to Sept.29
thu
Contents
K121013
ahpC
sufA
tetR
Data
K121013
n=1
10min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.373
0.310
0.290
0.293
0.328
0.272
0.317
0.338
0.372
0.3130
0.274
0.285
0.325
0.261
0.300
0.327
20min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.346
0.329
0.321
0.272
0.366
0.281
0.479
0.324
0.353
0.343
0.320
0.275
0.356
0.280
0.461
0.327
30min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.327
0.289
0.331
0.356
0.352
0.353
0.344
0.474
0.339
0.293
0.333
0.365
0.358
0.368
0.340
0.467
40min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.439
0.463
0.416
0.466
0.461
0.319
0.475
0.419
0.450
0.468
0.401
0.488
0.441
0.314
0.459
0.418
50min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.314
0.445
0.355
0.452
0.342
0.353
0.442
0.392
0.310
0.491
0.370
0.471
0.338
0.348
0.424
0.392
60min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.468
0.478
0.459
0.459
0.446
0.354
0.515
0.420
70min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.452
0.4480
0.453
0.510
0.414
0.456
0.416
0.435
0.434
0.434
0.521
0.400
0.421
0.428
0.402
0.423
80min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.439
0.458
0.450
0.417
0.510
0.248
0.401
0.428
0.427
0.430
0.426
0.413
0.477
0.248
0.401
0.432
K121013
H
2
O
2
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10min
0.373
0.311
0.281
0.289
0.326
0.266
0.307
0.333
20min
0.350
0.336
0.320
0.274
0.361
0.280
0.470
0.325
30min
0.333
0.291
0.332
0.361
0.355
0.361
0.342
0.471
40min
0.444
0.465
0.408
0.477
0.451
0.316
0.467
0.419
50min
0.312
0.468
0.363
0.462
0.340
0.351
0.433
0.392
60min
0.468
0.478
0.459
0.459
0.446
0.354
0.515
0.420
70min
0.443
0.441
0.487
0.455
0.417
0.442
0.409
0.429
80min
0.433
0.444
0.436
0.415
0.493
0.248
0.401
0.430
n=2
10min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.324
0.282
0.327
0.300
0.329
0.301
0.306
0.362
0.324
0.286
0.315
0.299
0.320
0.306
0.306
0.367
0.321
0.294
0.313
0.300
0.327
0.310
0.315
0.367
20min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.464
0.458
0.436
0.405
1.24
0.440
0.380
0.439
0.438
0.432
0.400
0.376
1.22
0.425
0.350
0.452
0.419
0.421
0.399
0.373
1.23
0.413
0.374
0.456
30min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.346
0.353
0.441
0.333
0.426
0.385
0.364
0.402
0.353
0.345
0.432
0.340
0.409
0.372
0.368
0.416
0.346
0.346
0.416
0.342
0.401
0.366
0.360
0.405
40min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.399
0.461
0.413
0.541
0.416
0.395
0.510
0.372
0.370
0.461
0.396
0.524
0.403
0.377
0.506
0.377
0.368
0.450
0.397
0.54
0.396
0.377
0.480
0.379
50min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.500
0.429
0.355
0.441
0.566
0.462
0.450
0.414
0.469
0.407
0.341
0.405
0.564
0.421
0.418
0.408
0.434
0.390
0.340
0.384
0.575
0.406
0.409
0.413
60min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.405
0.398
0.395
0.365
0.442
0.414
0.403
0.411
0.388
0.405
0.386
0.365
0.415
0.406
0.413
0.409
0.403
0.404
0.389
0.370
0.405
0.414
0.417
0.405
70min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.426
0.600
0.557
0.413
0.454
0.450
0.434
0.387
0.426
0.545
0.504
0.421
0.454
0.462
0.429
0.393
0.436
0.506
0.500
0.431
0.465
0.466
0.446
0.404
80min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.457
0.445
0.456
0.457
0.464
0.466
0.450
0.417
0.474
0.458
0.468
0.471
0.477
0.484
0.465
0.430
0.474
0.460
0.470
0.485
0.481
0.490
0.468
0.416
90min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.469
0.458
0.491
0.475
0.483
0.444
0.533
0.446
0.482
0.464
0.450
0.481
0.493
0.443
0.543
0.450
0.491
0.467
0.510
0.486
0.506
0.45
0.540
0.448
K121013
H
2
O
2
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10min
0.323
0.287
0.318
0.299
0.326
0.306
0.309
0.365
20min
0.440
0.437
0.412
0.385
1.23
0.426
0.368
0.449
30min
0.348
0.348
0.429
0.338
0.412
0.374
0.364
0.408
40min
0.468
0.409
0.345
0.410
0.568
0.430
0.425
0.412
50min
0.399
0.402
0.390
0.367
0.420
0.411
0.411
0.408
60min
0.429
0.550
0.520
0.422
0.457
0.459
0.436
0.395
70min
0.469
0.455
0.465
0.471
0.474
0.480
0.461
0.421
80min
0.481
0.463
0.483
0.481
0.494
0.446
0.539
0.448
n=3
10min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.674
0.662
0.684
0.663
0.723
0.646
0.667
0.682
0.675
0.667
0.679
0.667
0.723
0.655
0.659
0.698
0.679
0.662
0.682
0.661
0.731
0.661
0.647
0.693
20min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.715
0.704
0.712
0.705
0.695
0.696
0.768
1.06
0.715
0.690
0.698
0.706
0.694
0.695
0.753
1.04
0.714
0.708
0.705
0.699
0.709
0.701
0.736
1.04
30min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.809
0.770
0.664
0.704
0.759
0.686
0.844
0.726
0.792
0.760
0.659
0.715
0.759
0.692
0.806
0.717
0.811
0.775
0.681
0.711
0.758
0.699
0.816
0.716
40min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.768
0.729
0.713
0.704
0.800
0.790
0.776
0.742
0.766
0.726
0.703
0.710
0.796
0.788
0.769
0.750
0.772
0.731
0.707
0.717
0.802
0.785
0.773
0.759
50min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.722
0.729
0.755
0.784
0.814
0.842
0.838
0.918
0.733
0.732
0.759
0.786
0.820
0.848
0.843
0.930
0.740
0.735
0.767
0.793
0.825
0.846
0.838
0.936
60min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.901
0.885
0.981
0.866
0.834
0.888
0.844
0.968
0.908
0.897
0.988
0.873
0.819
0.895
0.835
0.963
0.914
0.898
0.985
0.887
0.843
0.881
0.833
0.956
70min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.942
0.985
0.967
0.917
0.914
0.910
0.970
0.932
0.941
0.972
0.955
0.900
0.911
0.897
0.940
0.931
0.942
0.966
0.957
0.900
0.903
0.879
0.953
0.941
80min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
1.04
1.01
0.994
1.02
1.03
1.01
0.995
1.17
1.02
1.01
0.987
1.01
0.969
0.995
0.974
1.14
1.03
1.02
1.01
1.00
0.976
0.999
0.979
1.14
90min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
0.979
1.01
1.05
1.02
1.04
0.989
0.933
1.12
0.997
1.01
1.06
1.01
0.995
1.01
0.951
1.11
0.982
1.01
1.07
1.04
1.02
1.00
0.949
1.10
K121013
H
2
O
2
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10min
0.676
0.663
0.682
0.664
0.726
0.654
0.658
0.691
20min
0.715
0.701
0.705
0.703
0.700
0.698
0.752
1.05
30min
0.804
0.768
0.668
0.710
0.759
0.693
0.822
0.720
40min
0.769
0.725
0.707
0.710
0.799
0.788
0.772
0.750
50min
0.732
0.732
0.760
0.787
0.820
0.845
0.840
0.928
60min
0.942
0.974
0.960
0.905
0.909
0.895
0.955
0.935
70min
1.03
1.02
0.997
1.01
0.990
1.00
0.983
1.15
80min
0.986
1.01
1.06
1.02
1.02
0.999
0.944
1.11
90min
0.986
1.01
1.06
1.03
1.02
0.999
0.944
1.11
ahpC
n=1
10min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
6.36
6.55
6.64
5.95
7.19
9.85
10.4
12.0
6.48
6.71
6.73
6.11
7.34
10.2
11.0
12.4
6.52
6.72
6.78
6.15
7.46
10.4
11.3
12.8
20min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
7.82
6.91
7.31
6.92
7.26
11.4
12.6
13.6
7.89
7.04
7.47
6.97
7.37
11.4
12.9
13.9
30min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
8.17
8.20
8.62
8.71
8.97
11.3
13.6
16.1
8.32
8.30
8.79
8.83
8.98
11.4
13.9
16.1
40min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
8.37
8.75
9.20
8.52
8.86
11.5
14.9
16.0
8.39
8.73
9.19
8.55
9.05
11.9
15.3
16.2
50min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10.3
10.5
11.5
11.1
11.9
11.1
16.6
19.8
10.5
10.8
11.8
11.5
12.3
11.7
17.2
20.2
60min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
11.9
13.3
13.0
13.3
13.8
16.0
19.4
22.5
12.1
13.7
13.3
13.5
14.0
16.3
19.8
23.1
70min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
16.7
16.0
15.8
15.7
18.0
21.2
24.2
25.0
17.2
16.6
16.4
16.0
18.5
21.5
24.9
25.2
80min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
21.2
27.0
22.2
23.4
23.5
25.6
28.9
31.3
22
27.7
22.8
24.1
24.1
25.7
29.1
31.6
ahpC
H
2
O
2
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10min
6.45
6.66
6.72
6.07
7.33
10.2
10.9
12.4
20min
7.86
6.98
7.39
6.94
7.32
11.4
12.8
13.7
30min
8.25
8.25
8.71
8.77
8.98
11.3
13.8
16.1
40min
8.38
8.74
9.20
8.53
8.95
11.7
15.1
16.1
50min
10.4
10.6
11.7
11.3
12.1
11.4
16.9
20.0
60min
12.0
13.5
13.2
13.4
13.9
16.1
19.6
22.8
70min
16.9
16.3
16.1
15.9
18.3
21.3
24.6
25.1
80min
16.9
16.3
16.1
15.9
18.3
21.3
24.6
25.1
n=2
10min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
7.33
7.58
7.41
7.47
8.27
8.71
10.8
10.7
7.32
7.53
7.30
7.35
8.26
8.49
11.3
11.6
7.34
7.52
7.28
7.29
8.26
8.48
11.5
11.8
20min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
8.05
8.17
8.03
8.19
9.05
10.7
11.4
13.2
8.19
8.27
8.19
8.54
9.21
10.9
11.6
13.5
8.34
8.35
8.33
8.57
9.31
11.1
11.8
13.8
30min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
8.70
8.65
8.79
8.76
10.7
11.1
12.4
14.5
8.89
8.76
8.97
8.84
10.7
10.9
12.5
14.6
8.96
8.94
9.10
8.91
10.8
11.1
12.9
15.0
40min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
9.36
9.20
8.83
9.08
10.5
12.7
13.2
15.8
9.60
9.38
8.96
9.20
10.6
12.8
3.44
15.9
9.69
9.40
9.12
9.32
10.8
12.8
13.6
15.9
50min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
10.1
10.5
10.3
10.2
11.3
12.8
14.6
17.9
10.5
10.9
10.6
10.7
11.5
13.1
14.8
18.2
10.7
11.1
10.8
10.8
11.8
13.4
15.1
18.4
60min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
11.2
12.0
11.9
12.5
12.8
14.4
16.5
19.2
11.3
12.2
12.1
12.7
13.1
14.5
16.6
19.2
11.5
12.5
12.4
13.1
13.3
14.9
16.9
19.3
70min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
13.2
12.3
13.6
13.4
15.8
17.6
18.4
21.3
12.8
12.3
13.3
13.5
15.4
17.4
18.0
20.5
12.9
13.1
14.2
13.7
16.1
17.9
18.9
20.9
80min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
16.2
18.1
17.9
18.3
18.3
20.9
17.5
22.9
16.1
18.2
17.9
18.5
18.1
20.5
17.3
22.3
16.4
18.9
18.4
19.3
18.7
21.1
18.2
23.4
ahpC
H
2
O
2
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
10min
7.33
7.54
7.33
7.37
8.26
8.56
11.2
11.4
20min
8.20
8.26
8.18
8.43
9.19
10.9
11.6
13.5
30min
8.85
8.78
8.95
8.84
10.7
11.0
12.6
14.7
40min
9.55
9.33
8.97
9.20
10.6
12.8
13.4
15.8
50min
10.4
10.8
10.6
10.6
11.5
13.1
14.8
18.2
60min
11.3
12.2
12.2
12.8
13.1
14.6
16.7
19.2
70min
13.0
12.5
13.7
13.5
15.8
17.6
18.4
20.9
80min
16.3
18.4
18.1
18.7
18.4
20.8
17.7
22.9
n=3
10min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
5.52
5.37
5.72
5.53
6.00
6.93
7.63
7.77
5.63
5.46
5.69
5.49
5.95
6.93
7.67
7.71
5.56
5.42
5.69
5.44
5.95
6.97
7.80
7.80
20min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
6.11
5.92
5.70
5.54
5.77
7.36
8.28
10.0
6.05
5.89
5.50
5.43
5.57
7.17
7.96
9.71
6.08
5.93
5.55
5.51
5.67
7.28
8.22
9.98
30min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
6.02
5.81
6.29
6.03
6.30
7.78
8.56
10.1
6.03
5.81
6.26
6.03
6.29
7.42
8.52
9.96
6.12
5.87
6.31
6.10
6.35
7.56
8.63
9.95
40min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
6.22
5.90
6.46
6.01
5.74
12.2
8.66
10.3
6.30
6.07
6.61
6.20
5.83
12.7
8.89
10.4
6.39
6.15
6.66
6.32
6.00
12.9
9.10
10.5
50min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
6.59
6.48
6.77
6.74
6.92
8.14
9.59
12.1
6.72
6.66
6.95
6.90
7.00
8.33
9.74
12.1
6.86
6.80
7.10
7.05
7.11
8.47
10.0
12.3
60min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
6.36
7.38
7.05
7.15
7.84
8.82
10.4
11.7
6.43
7.60
7.25
7.38
7.94
8.85
10.5
11.8
6.71
7.79
7.42
7.56
8.10
9.02
10.6
11.9
70min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
9.19
9.16
9.25
9.09
8.83
11.7
12.5
13.0
9.40
9.31
9.21
9.15
8.71
11.5
12.4
13.3
9.61
9.56
9.37
9.36
8.71
11.8
12.6
13.4
80min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
11.0
11.1
11.5
10.9
12.0
13.7
15.4
15.4
11.2
11.3
11.6
11.1
12.2
13.5
15.2
15.3
11.3
11.7
11.8
11.6
12.4
13.9
15.8
15.4
90min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
16.0
17.6
15.0
14.6
15.3
16.4
17.1
17.3
16.2
17.8
15.1
14.7
15.5
16.4
17.2
17.5
16.4
18.1
15.3
15.0
15.7
16.6
17.4
17.7
sufA
n=1
10min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
1.24
1.13
1.33
1.26
1.29
1.49
2.62
4.24
1.278
1.16
1.34
1.29
1.27
1.52
2.74
4.28
1.29
1.16
1.33
1.29
1.27
1.55
2.75
4.36
20min
1.34
1.27
1.39
1.32
1.40
1.53
3.07
5.20
1.34
1.25
1.38
1.32
1.43
1.56
3.08
5.09
30min
1.31
1.25
1.39
1.30
1.34
1.65
2.91
5.03
1.33
1.29
1.41
1.32
1.37
1.67
2.94
4.97
40min
1.40
1.41
1.39
1.36
1.42
1.62
3.07
5.31
1.38
1.38
1.37
1.34
1.39
1.64
3.17
5.27
50min
1.72
0.983
1.23
1.38
1.40
1.53
3.17
5.49
1.23
0.994
1.26
1.35
1.39
1.59
3.17
5.58
60min
1.37
1.50
1.62
1.23
1.58
1.90
3.40
5.85
1.43
1.58
1.69
1.27
1.61
1.92
3.49
5.92
70min
1.77
1.77
2.46
1.82
2.11
2.20
4.00
6.13
1.90
1.87
2.58
1.92
2.18
2.24
4.00
6.10
80min
2.81
2.76
2.86
2.84
1.80
3.20
5.44
3.32
3.07
3.01
2.17
3.01
1.91
3.29
5.52
3.26
sufA
H
2
O
2
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
10min
1.27
1.15
1.33
1.28
1.28
1.52
2.70
4.29
20min
1.34
1.26
1.38
1.32
1.42
1.54
3.07
5.15
30min
1.32
1.27
1.40
1.31
1.35
1.66
2.92
5.00
40min
1.39
1.39
1.38
1.35
1.40
1.63
3.12
5.29
50min
1.47
0.989
1.24
1.36
1.40
1.56
3.17
5.53
60min
1.40
1.54
1.65
1.25
1.60
1.91
3.45
5.89
70min
1.84
1.82
2.52
1.87
2.14
2.22
3.99
6.11
80min
2.94
2.89
2.51
2.93
1.86
3.24
5.48
3.29
n=2
10min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
3.03
3.12
3.16
2.96
3.15
3.12
3.49
3.03
3.05
3.10
2.93
3.10
3.08
3.54
4.07
3.01
3.07
3.13
2.95
3.11
3.06
3.46
4.07
20min
3.11
3.10
2.94
2.99
3.48
2.95
3.67
4.41
3.07
3.06
2.91
2.57
3.46
3.00
3.65
4.52
3.03
3.10
2.94
3.05
3.46
2.98
3.89
4.43
30min
3.30
1.93
3.28
3.00
3.19
3.18
3.80
4.57
3.21
1.81
3.16
2.98
3.15
3.15
3.74
4.59
3.17
1.90
3.16
3.03
3.22
3.17
3.80
4.67
40min
3.09
3.15
2.97
2.82
3.14
3.12
3.48
4.53
3.10
3.26
3.00
2.88
3.15
3.14
3.60
4.51
3.07
3.26
3.01
2.90
3.14
3.17
3.63
4.54
50min
3.15
3.06
3.06
3.10
3.14
3.37
3.78
4.84
3.11
3.11
3.13
3.18
3.25
3.39
3.86
4.84
3.11
3.10
3.09
3.14
3.22
3.37
3.82
4.83
60min
3.07
3.11
3.07
3.38
3.21
3.31
3.69
4.84
3.00
3.10
3.05
3.41
3.27
3.39
3.75
4.93
3.01
3.14
3.07
3.45
3.26
3.38
3.73
5.01
70min
3.64
3.29
3.37
3.52
3.33
3.41
4.08
4.99
3.51
3.11
3.21
3.36
3.14
3.26
3.37
4.75
3.59
3.12
3.27
3.40
3.19
3.26
3.78
4.69
80min
3.79
3.28
4.29
3.07
3.27
3.39
4.04
4.81
3.77
3.29
4.28
3.03
3.22
3.39
4.01
4.71
3.76
3.30
4.27
3.04
3.26
3.45
3.99
4.82
sufA
H
2
O
2
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
10min
3.02
3.08
3.13
2.95
3.12
3.09
3.50
4.11
20min
3.07
3.09
2.93
2.87
3.47
2.98
3.73
4.45
30min
3.23
1.88
3.21
3.01
3.18
3.17
3.78
4.61
40min
3.09
3.22
2.99
2.87
3.15
3.14
3.57
4.53
50min
3.12
3.09
3.09
3.14
3.20
3.38
3.82
4.84
60min
3.02
3.12
3.07
3.41
3.24
3.36
3.72
4.93
70min
3.58
3.17
3.28
3.43
3.22
3.31
3.74
4.81
80min
3.77
3.29
4.28
3.05
3.25
3.41
4.01
4.78
n=3
10min
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
2.10
1.87
2.03
2.09
2.08
2.15
2.82
3.49
2.07
1.87
1.99
2.07
2.00
2.12
2.76
3.51
2.02
1.85
1.98
2.05
1.98
2.12
2.81
3.59
20min
2.29
2.62
2.24
2.07
2.03
2.15
2.83
4.25
2.24
2.00
2.20
2.08
2.01
2.14
2.86
4.19
2.25
2.02
2.19
2.09
2.02
2.16
2.88
4.29
30min
2.04
1.95
1.89
1.96
2.09
2.07
2.90
4.09
2.00
1.86
1.87
1.96
2.06
2.09
2.96
4.09
2.01
1.91
1.91
1.98
2.08
2.11
2.99
4.10
40min
1.95
1.93
2.16
2.04
2.06
2.10
2.70
4.33
1.94
1.87
2.17
2.06
2.08
2.15
2.81
4.34
1.95
1.88
2.21
2.09
2.07
2.18
2.84
4.36
50min
1.92
2.06
2.03
2.06
1.93
2.23
2.63
4.63
1.92
2.09
2.04
2.09
1.94
2.22
2.67
4.62
1.92
2.07
2.06
2.10
2.08
2.22
2.70
4.64
60min
1.83
1.96
2.09
2.03
2.02
2.26
2.75
4.65
1.84
1.99
2.12
2.05
2.03
2.25
2.80
4.65
1.87
2.00
2.12
2.08
2.02
2.27
2.77
4.67
70min
2.13
2.22
2.22
2.09
2.23
2.30
2.90
4.32
2.12
2.21
2.22
2.04
2.22
2.26
2.85
4.26
2.15
2.24
2.24
2.07
2.23
2.28
2.86
4.26
80min
2.16
2.20
2.25
1.97
2.24
2.29
2.87
4.45
2.15
2.21
2.23
1.94
2.23
2.28
2.84
4.37
2.17
2.22
2.27
1.97
2.25
2.27
2.85
4.36
90min
2.29
2.13
2.26
2.16
2.19
2.28
3.08
4.30
2.30
2.16
2.27
2.17
2.13
2.29
3.08
4.31
2.28
2.19
2.30
2.20
2.16
2.31
3.14
4.38
sufA
H
2
O
2
less
1nM
10nM
100nM
1μM
10μM
100μM
1mM
10min
2.06
1.86
2.00
2.07
2.02
2.13
2.80
3.53
20min
2.26
2.21
2.21
2.08
2.02
2.15
2.86
4.24
30min
2.01
1.91
1.89
1.97
2.08
2.09
2.95
4.09
40min
1.95
1.89
2.18
2.07
2.07
2.14
2.78
4.34
50min
1.92
2.07
2.05
2.08
1.98
2.23
2.67
4.63
60min
1.85
1.98
2.11
2.05
2.02
2.26
2.77
4.65
70min
2.13
2.22
2.23
2.06
2.22
2.28
2.87
4.28
80min
2.16
2.21
2.25
1.96
2.24
2.28
2.85
4.39
90min
2.29
2.14
2.28
2.18
2.16
2.30
3.10
4.33
tetR
n=1
10min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
14.4
14.63
14.28
15.01
14.66
14.97
14.96
13.84
14.51
14.76
14.3
15.01
14.52
15.04
14.78
13.82
20min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
15.19
15.24
15.03
15.99
14.85
14.7
14.79
15.09
15.5
15.46
15.21
15.17
14.78
14.75
14.57
14.71
30min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
15.67
15.94
15.99
16.32
15.23
16.5
16.34
15.43
16.02
16.21
16.29
16.41
15.34
16.57
16.47
15.64
40min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
13.32
15.19
14.39
14.48
14.82
15.58
15.51
15.26
14.35
16.09
15.03
14.95
15.47
15.8
15.88
15.42
50min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
18.08
18.31
17.4
18.41
17.03
18.25
18.18
19.67
18.26
18.61
17.78
18.72
17.4
18.51
18.53
20.16
60min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
18.46
19.82
17.61
18.28
18.69
19.21
18.96
21.1
70min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
21.83
21.43
20.92
20.81
21.89
21.8
20.7
20.2
21.93
21.27
20.72
20.76
21.9
21.49
20.62
20.09
80min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
22.89
25.21
22.64
22.08
21.57
22.78
22.14
20.79
23.18
24.76
22.76
22.09
21.79
22.85
22.4
20.84
tetR
H
2
O
2
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10min
14.455
14.695
14.29
15.01
14.59
15.005
14.87
13.83
20min
15.345
15.35
15.12
15.58
14.815
14.725
14.68
14.9
30min
15.845
16.075
16.14
16.365
15.285
16.535
16.405
15.535
40min
13.835
15.64
14.71
14.715
15.145
15.69
15.695
15.34
50min
18.17
18.46
17.59
18.565
17.215
18.38
18.355
19.915
60min
18.46
19.82
17.61
18.28
18.69
19.21
18.96
21.1
70min
21.88
21.35
20.82
20.785
21.895
21.645
20.66
20.145
80min
23.035
24.985
22.7
22.085
21.68
22.815
22.27
20.815
n=2
10min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
26.22
26.79
26.21
26.37
21.76
25.34
25.45
24.3
26.15
26.58
25.88
26.06
21.61
25.83
26.38
24.5
26.12
26.78
26.11
26.17
22
26.29
26.38
24.8
20min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
28.89
28.05
24.45
26.77
26.75
27.74
25.68
24.98
29.39
27.76
24.48
26.63
26.43
27.31
25.49
25.44
29.83
27.96
24.93
27.11
26.66
27.7
25.92
25.79
30min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
28.69
28
28.99
28.11
27.02
27.41
27.93
28.36
29.32
28.52
29.56
28.57
27.31
28.06
27.75
28.29
29.73
28.8
29.95
28.88
27.89
28.34
28.19
28.15
40min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
26.57
26.86
19.84
27.5
29.31
27.13
26.25
28.5
27.66
28.26
20.75
29.22
30.63
29.28
26.95
27.8
28.24
28.99
20.94
29.16
31.2
29.48
27.29
27.98
50min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
32.92
29.34
29.62
29.62
31.46
31.56
31.3
30.59
32.97
29.61
29.91
29.7
31.7
32.03
31.48
30.73
33.04
29.95
30.21
30.15
32.01
32.34
31.78
30.91
60min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
34.98
29.12
28.81
26.91
26.78
27.07
29.59
27.92
34.87
27.12
28.81
26.91
26.78
27.07
29.59
27.93
34.71
27.77
28.64
26.94
26.39
27.42
29.64
27.85
70min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
37.86
34.52
33.63
33
32.38
32.36
31.13
33.74
37.84
34.55
33.61
33.17
32.73
32.16
31.35
33.6
38.25
35.34
34.1
33.48
33.07
33.08
31.94
34.06
80min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
38.47
35.23
36.74
35.94
38.05
36.91
32.52
34.6
38.92
35.85
37.22
36.79
38.96
37.85
33.18
35.29
39.4
36.3
37.34
36.97
39.09
38.12
33.38
35.39
90min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
40.1
38.61
37.85
36.69
37.09
37.14
38.34
34.36
40.45
38.81
38.51
36.73
37.36
38.45
39
35.2
40.69
39.17
38.96
37.29
37.5
38.59
39.37
35.66
tetR
H
2
O
2
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10min
26.16333333
26.71666667
26.06666667
26.2
21.79
25.82
26.07
24.53333333
20min
29.37
27.92333333
24.62
26.83666667
26.61333333
27.58333333
25.69666667
25.40333333
30min
29.24666667
28.44
29.5
28.52
27.40666667
27.93666667
27.95666667
28.26666667
40min
32.97666667
29.63333333
29.91333333
29.82333333
31.72333333
31.97666667
31.52
30.74333333
50min
34.85333333
28.00333333
28.75333333
26.92
26.65
27.18666667
29.60666667
27.9
60min
37.98333333
34.80333333
33.78
33.21666667
32.72666667
32.53333333
31.47333333
33.8
70min
38.93
35.79333333
37.1
36.56666667
38.7
37.62666667
33.02666667
35.09333333
80min
40.41333333
38.86333333
38.44
36.90333333
37.31666667
38.06
38.90333333
35.07333333
n=3
10min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
28.34
30.57
28.49
27.78
29.57
33.59
31.75
24.05
28.86
30.27
28.14
27.62
29.56
33.47
32
24.32
28.85
30.28
27.99
27.4
29.51
33.46
32.08
24.24
20min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
33.47
30.16
30.2
31.18
33.16
31.41
31.29
31.16
33.35
30.04
29.86
30.84
32.89
31.04
31.28
31.16
33.35
30.41
29.91
30.93
32.76
31.28
31.46
31.23
30min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
33.51
34.01
34.76
25.79
30.31
35.69
30.96
32.08
33.27
33.93
34.8
25.64
30.45
35.36
31.23
31.96
33.2
34.14
34.88
26.1
30.62
36.26
31.61
32.04
40min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
30.93
33.97
36.09
34.17
34.12
31.52
31.59
32.94
30.97
33.99
36.54
34.46
34.75
31.36
32.38
33.21
31.47
34.35
37.08
35.43
35.35
32.42
33.12
33.7
50min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
34.23
35.27
36.04
35.6
34.95
35.64
37.32
36.38
34.61
35.82
36.59
36.5
35.63
36.28
37.97
36.82
35.11
36.12
36.95
36.86
35.97
36.84
38.34
37.04
60min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
37.42
35.45
33.04
36.98
38.2
34.65
36.73
34.11
37.56
35.95
33.27
37.99
39.03
35.21
37.28
34.55
38.25
36.54
33.66
38.03
39.5
35.56
37.93
35.24
70min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
41.5
42.76
39.97
40.66
39.42
37.88
37.23
36.71
41.58
42.3
40.24
40.7
40.28
38.56
38
37.41
41.84
42.49
40.09
40.87
40.46
39.06
38.43
37.74
80min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
41.39
39.83
43.44
42.75
41.89
42.33
43.18
39.6
41.63
39.9
43.88
43.03
42.48
43.44
43.05
39.62
41.92
40.22
44.26
43.3
42.92
43.98
43.63
39.93
90min
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
41.78
39.67
40.24
39.2
39.94
38.86
36.98
35.74
41.9
39.66
40.01
38.82
39.6
38.31
36.74
35.95
41.92
39.65
40.3
38.84
39.81
38.64
37.16
35.87
tetR
H
2
O
2
less
1nM
10nM
100nM
1µM
10µM
100µM
1mM
10min
28.68333333
30.37333333
28.20666667
27.6
29.54666667
33.50666667
31.94333333
24.20333333
20min
33.39
30.20333333
29.99
30.98333333
32.93666667
31.24333333
31.34333333
31.18333333
30min
33.32666667
34.02666667
34.81333333
25.84333333
30.46
35.77
31.26666667
32.02666667
40min
31.12333333
34.10333333
36.57
34.68666667
34.74
31.76666667
32.36333333
33.28333333
50min
34.65
35.73666667
36.52666667
36.32
35.51666667
36.25333333
37.87666667
36.74666667
60min
41.64
42.51666667
40.1
40.74333333
40.05333333
38.5
37.88666667
37.28666667
70min
41.64666667
39.98333333
43.86
43.02666667
42.43
43.25
43.28666667
39.71666667
80min
41.86666667
39.66
40.18333333
38.95333333
39.78333333
38.60333333
36.96
35.85333333
90min
41.86666667
39.66
40.18333333
38.95333333
39.78333333
38.60333333
36.96
35.85333333