Team:KIT-Kyoto/Notebook/Promoters

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各種プロモーター活性

>>retuen to Sept.29^th

Contents

Data

K121013

n=1
10min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.373	0.310	0.290	0.293	0.328	0.272	0.317	0.338
	0.3720	0.3130	0.274	0.285	0.325	0.261	0.300	0.327

20min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.346	0.329	0.321	0.272	0.366	0.281	0.479	0.324
	0.353	0.343	0.320	0.275	0.356	0.280	0.461	0.327

30min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.327	0.289	0.331	0.356	0.352	0.353	0.344	0.474
	0.339	0.293	0.333	0.365	0.358	0.368	0.340	0.467

40min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.439	0.463	0.416	0.466	0.461	0.319	0.475	0.419
	0.450	0.468	0.401	0.488	0.441	0.314	0.459	0.418

50min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.314	0.445	0.355	0.452	0.342	0.353	0.442	0.392
	0.310	0.491	0.370	0.471	0.338	0.348	0.424	0.392

60min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.468	0.478	0.459	0.459	0.446	0.354	0.515	0.420

70min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.452	0.4480	0.453	0.510	0.414	0.456	0.416	0.435
	0.434	0.434	0.521	0.400	0.421	0.428	0.402	0.423

80min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.439	0.458	0.450	0.417	0.510	0.248	0.401	0.428
	0.427	0.430	0.426	0.413	0.477	0.248	0.401	0.432

K121013	H₂O₂
	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
10min	0.373	0.311	0.281	0.289	0.326	0.266	0.307	0.333
20min	0.350	0.336	0.320	0.274	0.361	0.280	0.470	0.325
30min	0.333	0.291	0.332	0.361	0.355	0.361	0.342	0.471
40min	0.444	0.465	0.408	0.477	0.451	0.316	0.467	0.419
50min	0.312	0.468	0.363	0.462	0.340	0.351	0.433	0.392
60min	0.468	0.478	0.459	0.459	0.446	0.354	0.515	0.420
70min	0.443	0.441	0.487	0.455	0.417	0.442	0.409	0.429
80min	0.433	0.444	0.436	0.415	0.493	0.248	0.401	0.430

n=2

10min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.324	0.282	0.327	0.300	0.329	0.301	0.306	0.362
	0.324	0.286	0.315	0.299	0.320	0.306	0.306	0.367
	0.321	0.294	0.313	0.300	0.327	0.310	0.315	0.367

20min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.464	0.458	0.436	0.405	1.24	0.440	0.380	0.439
	0.438	0.432	0.400	0.376	1.22	0.425	0.350	0.452
	0.419	0.421	0.399	0.373	1.23	0.413	0.374	0.456

30min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.346	0.353	0.441	0.333	0.426	0.385	0.364	0.402
	0.353	0.345	0.432	0.340	0.409	0.372	0.368	0.416
	0.346	0.346	0.416	0.342	0.401	0.366	0.360	0.405

40min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.399	0.461	0.413	0.541	0.416	0.395	0.510	0.372
	0.370	0.461	0.396	0.524	0.403	0.377	0.506	0.377
	0.368	0.450	0.397	0.54	0.396	0.377	0.480	0.379

50min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.500	0.429	0.355	0.441	0.566	0.462	0.450	0.414
	0.469	0.407	0.341	0.405	0.564	0.421	0.418	0.408
	0.434	0.390	0.340	0.384	0.575	0.406	0.409	0.413

60min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.405	0.398	0.395	0.365	0.442	0.414	0.403	0.411
	0.388	0.405	0.386	0.365	0.415	0.406	0.413	0.409
	0.403	0.404	0.389	0.370	0.405	0.414	0.417	0.405

70min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.426	0.600	0.557	0.413	0.454	0.450	0.434	0.387
	0.426	0.545	0.504	0.421	0.454	0.462	0.429	0.393
	0.436	0.506	0.500	0.431	0.465	0.466	0.446	0.404

80min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.457	0.445	0.456	0.457	0.464	0.466	0.450	0.417
	0.474	0.458	0.468	0.471	0.477	0.484	0.465	0.430
	0.474	0.460	0.470	0.485	0.481	0.490	0.468	0.416

90min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.469	0.458	0.491	0.475	0.483	0.444	0.533	0.446
	0.482	0.464	0.450	0.481	0.493	0.443	0.543	0.450
	0.491	0.467	0.510	0.486	0.506	0.45	0.540	0.448

K121013	H₂O₂
	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
10min	0.323	0.287	0.318	0.299	0.326	0.306	0.309	0.365
20min	0.440	0.437	0.412	0.385	1.23	0.426	0.368	0.449
30min	0.348	0.348	0.429	0.338	0.412	0.374	0.364	0.408
40min	0.468	0.409	0.345	0.410	0.568	0.430	0.425	0.412
50min	0.399	0.402	0.390	0.367	0.420	0.411	0.411	0.408
60min	0.429	0.550	0.520	0.422	0.457	0.459	0.436	0.395
70min	0.469	0.455	0.465	0.471	0.474	0.480	0.461	0.421
80min	0.481	0.463	0.483	0.481	0.494	0.446	0.539	0.448

n=3

10min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.674	0.662	0.684	0.663	0.723	0.646	0.667	0.682
	0.675	0.667	0.679	0.667	0.723	0.655	0.659	0.698
	0.679	0.662	0.682	0.661	0.731	0.661	0.647	0.693

20min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.715	0.704	0.712	0.705	0.695	0.696	0.768	1.06
	0.715	0.690	0.698	0.706	0.694	0.695	0.753	1.04
	0.714	0.708	0.705	0.699	0.709	0.701	0.736	1.04

30min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.809	0.770	0.664	0.704	0.759	0.686	0.844	0.726
	0.792	0.760	0.659	0.715	0.759	0.692	0.806	0.717
	0.811	0.775	0.681	0.711	0.758	0.699	0.816	0.716

40min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.768	0.729	0.713	0.704	0.800	0.790	0.776	0.742
	0.766	0.726	0.703	0.710	0.796	0.788	0.769	0.750
	0.772	0.731	0.707	0.717	0.802	0.785	0.773	0.759

50min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.722	0.729	0.755	0.784	0.814	0.842	0.838	0.918
	0.733	0.732	0.759	0.786	0.820	0.848	0.843	0.930
	0.740	0.735	0.767	0.793	0.825	0.846	0.838	0.936

60min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.901	0.885	0.981	0.866	0.834	0.888	0.844	0.968
	0.908	0.897	0.988	0.873	0.819	0.895	0.835	0.963
	0.914	0.898	0.985	0.887	0.843	0.881	0.833	0.956

70min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.942	0.985	0.967	0.917	0.914	0.910	0.970	0.932
	0.941	0.972	0.955	0.900	0.911	0.897	0.940	0.931
	0.942	0.966	0.957	0.900	0.903	0.879	0.953	0.941

80min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	1.04	1.01	0.994	1.02	1.03	1.01	0.995	1.17
	1.02	1.01	0.987	1.01	0.969	0.995	0.974	1.14
	1.03	1.02	1.01	1.00	0.976	0.999	0.979	1.14

90min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.979	1.01	1.05	1.02	1.04	0.989	0.933	1.12
	0.997	1.01	1.06	1.01	0.995	1.01	0.951	1.11
	0.982	1.01	1.07	1.04	1.02	1.00	0.949	1.10

K121013	H₂O₂
	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
10min	0.676	0.663	0.682	0.664	0.726	0.654	0.658	0.691
20min	0.715	0.701	0.705	0.703	0.700	0.698	0.752	1.05
30min	0.804	0.768	0.668	0.710	0.759	0.693	0.822	0.720
40min	0.769	0.725	0.707	0.710	0.799	0.788	0.772	0.750
50min	0.732	0.732	0.760	0.787	0.820	0.845	0.840	0.928
60min	0.942	0.974	0.960	0.905	0.909	0.895	0.955	0.935
70min	1.03	1.02	0.997	1.01	0.990	1.00	0.983	1.15
80min	0.986	1.01	1.06	1.02	1.02	0.999	0.944	1.11
90min	0.986	1.01	1.06	1.03	1.02	0.999	0.944	1.11

ahpC

n=1

10min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	6.36	6.55	6.64	5.95	7.19	9.85	10.4	12.0
	6.48	6.71	6.73	6.11	7.34	10.2	11.0	12.4
	6.52	6.72	6.78	6.15	7.46	10.4	11.3	12.8

20min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	7.82	6.91	7.31	6.92	7.26	11.4	12.6	13.6
	7.89	7.04	7.47	6.97	7.37	11.4	12.9	13.9

30min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	8.17	8.20	8.62	8.71	8.97	11.3	13.6	16.1
	8.32	8.30	8.79	8.83	8.98	11.4	13.9	16.1

40min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	8.37	8.75	9.20	8.52	8.86	11.5	14.9	16.0
	8.39	8.73	9.19	8.55	9.05	11.9	15.3	16.2

50min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	10.3	10.5	11.5	11.1	11.9	11.1	16.6	19.8
	10.5	10.8	11.8	11.5	12.3	11.7	17.2	20.2

60min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	11.9	13.3	13.0	13.3	13.8	16.0	19.4	22.5
	12.1	13.7	13.3	13.5	14.0	16.3	19.8	23.1

70min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	16.7	16.0	15.8	15.7	18.0	21.2	24.2	25.0
	17.2	16.6	16.4	16.0	18.5	21.5	24.9	25.2

80min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	21.2	27.0	22.2	23.4	23.5	25.6	28.9	31.3
	22	27.7	22.8	24.1	24.1	25.7	29.1	31.6

ahpC	H₂O₂
	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
10min	6.45	6.66	6.72	6.07	7.33	10.2	10.9	12.4
20min	7.86	6.98	7.39	6.94	7.32	11.4	12.8	13.7
30min	8.25	8.25	8.71	8.77	8.98	11.3	13.8	16.1
40min	8.38	8.74	9.20	8.53	8.95	11.7	15.1	16.1
50min	10.4	10.6	11.7	11.3	12.1	11.4	16.9	20.0
60min	12.0	13.5	13.2	13.4	13.9	16.1	19.6	22.8
70min	16.9	16.3	16.1	15.9	18.3	21.3	24.6	25.1
80min	16.9	16.3	16.1	15.9	18.3	21.3	24.6	25.1

n=2

10min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	7.333	7.58	7.411	7.467	8.268	8.713	10.84	10.7
	7.317	7.53	7.296	7.348	8.263	8.492	11.3	11.55
	7.335	7.521	7.279	7.294	8.257	8.478	11.51	11.82

20min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	8.05	8.174	8.031	8.194	9.053	10.74	11.36	13.23
	8.194	8.268	8.191	8.535	9.21	10.92	11.62	13.46
	8.343	8.346	8.33	8.573	9.305	11.07	11.83	13.77

30min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	8.695	8.646	8.785	8.764	10.66	11.09	12.42	14.49
	8.888	8.759	8.966	8.837	10.69	10.89	12.5	14.62
	8.961	8.943	9.097	8.908	10.82	11.08	12.9	14.99

40min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	9.358	9.202	8.825	9.08	10.45	12.69	13.23	15.75
	9.595	9.378	8.961	9.202	10.6	12.83	3.44	15.86
	9.688	9.403	9.116	9.324	10.77	12.77	13.63	15.91

50min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	10.13	10.52	10.3	10.19	11.25	12.81	14.59	17.94
	10.49	10.86	10.63	10.65	11.54	13.09	14.83	18.18
	10.69	11.09	10.84	10.81	11.81	13.36	15.08	18.36

60min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	11.16	11.96	11.93	12.54	12.82	14.43	16.53	19.15
	11.26	12.23	12.11	12.71	13.05	14.48	16.62	19.21
	11.54	12.45	12.41	13.07	13.3	14.92	16.91	19.32

70min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	13.23	12.29	13.61	13.44	15.82	17.61	18.35	21.25
	12.76	12.25	13.28	13.46	15.42	17.38	18.01	20.47
	12.91	13.05	14.17	13.74	16.12	17.89	18.87	20.93

80min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	16.23	18.13	17.86	18.3	18.33	20.91	17.46	22.93
	16.11	18.24	17.9	18.53	18.05	20.51	17.34	22.31
	16.44	18.88	18.4	19.29	18.69	21.1	18.15	23.41

ahpC	H₂O₂
	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
10min	7.328333333	7.543666667	7.328666667	7.369666667	8.262666667	8.561	11.21666667	11.35666667
20min	8.195666667	8.262666667	8.184	8.434	9.189333333	10.91 11.60333333	13.48666667
30min	8.848	8.782666667	8.949333333	8.836333333	10.72333333	11.02	12.60666667	14.7
40min	9.547	9.327666667	8.967333333	9.202	10.60666667	12.76333333	13.43333333	15.84
50min	10.43666667	10.82333333	10.59	10.55	11.53333333	13.08666667	14.83333333	18.16
60min	11.32	12.21333333	12.15	12.77333333	13.05666667	14.61	16.68666667	19.22666667
70min	12.96666667	12.53	13.68666667	13.54666667	15.78666667	17.62666667	18.41	20.88333333
80min	16.26	18.41666667	18.05333333	18.70666667	18.35666667	20.84	17.65	22.88333333

n=3

10min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
5.519	5.366	5.722	5.526	6.003	6.93	7.826	7.772
5.628	5.463	5.693	5.491	5.948	6.931	7.672	7.709
5.555	5.419	5.69	5.439	5.948	6.97	7.796	7.799

20min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	6.114	5.922	5.68	5.54	5.765	7.362	8.28	10
	6.045	5.887	5.504	5.428	5.571	7.169	7.957	9.709
	6.076	5.928	5.551	5.51	5.665	7.278	8.215	9.976

30min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	6.02	5.805	6.293	6.025	6.297	7.475	8.558	10.12
	6.027	5.805	6.261	6.028	6.286	7.421	8.522	9.961
	6.116	5.871	6.314	6.102	6.349	7.561	8.628	9.952

40min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	6.217	5.9	6.459	6.009	5.743	12.2	8.663	10.28
	6.302	6.069	6.606	6.202	5.831	12.71	8.892	10.38
	6.392	6.145	6.663	6.317	6.002	12.94	9.101	10.52

50min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	6.59	6.478	6.767	6.743	6.917	8.139	9.588	12.06
	6.724	6.662	6.953	6.901	7	8.334	9.738	12.09
	6.859	6.801	7.103	7.051	7.109	8.47	10.04	12.34

60min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	6.356	7.38	7.051	7.147	7.835	8.82	10.36	11.71
	6.433	7.603	7.245	7.381	7.938	8.852	10.47	11.75
	6.707	7.79	7.424	7.559	8.102	9.022	10.63	11.9

70min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	9.194	9.162	9.247	9.085	8.83	11.65	12.54	12.95
	9.395	9.308	9.208	9.154	8.706	11.53	12.44	13.27
	9.605	9.564	9.367	9.358	8.712	11.78	12.62	13.42

80min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	10.99	11.08	11.51	10.92	12.04	13.71	15.42	15.37
	11.17	11.32	11.64	11.07	12.24	13.5	15.22	15.26
	11.34	11.67	11.84	11.64	12.37	13.85	15.8	15.35

90min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	16.03	17.62	14.97	14.62	15.31	16.37	17.11	17.3
	16.2	17.81	15.08	14.67	15.46	16.44	17.19	17.45
	16.39	18.07	15.34	14.96	15.7	16.64	17.38	17.66

sufA

tetR

Retrieved from "http://2010.igem.org/Team:KIT-Kyoto/Notebook/Promoters"