Team:Paris Liliane Bettencourt/Notebook/2010/08/18/
From 2010.igem.org
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Raphaël and Aleksandra
Test population counter (PsS/attC + PBad/Int 4))
2 conditions :
- 1/2 dilution from the overnight culture -> 10h induction, 0,2% arabinose
- 1/50 dilution from the overnight culture -> 10h induction, 0,2% arabinose
-> After 10h, plating with 1/100 and 1/1000 dilutions (there was just few red colonies on the plates, it's impossible to calculate the recombination rate because the constructs are in a high copy plasmid)
Léa
Minipreps
- 2)Ps/wRBS/LuxR + wRBS/TetA(C)/LuxI, 4 colonies (1 didn't grow)
- 6)Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI, 4 colonies
- 8)Ps/sRBS/LuxR + mRBS/TetA(C)/LuxI, 1 colony
- 9)Pm/sRBS/LuxR + sRBS/TetA(C)/LuxI, 1 colony
- 10)Ps/wRBS/LuxR + sRBS/TetA(C)/LuxI, 4 colonies
- 11)Ps/mRBS/LuxR + sRBS/TetA(C)/LuxI, 4 colonies (2 didn't grow)
- wRBS/LacZα/LuxI, 4 colonies
- sRBS/LacZα/LuxI, 4 colonies
- LuxI
- pLux/LuxI, 3 colonies
- PmW/attC, 1 colony
-> We couldn't use the nanodrop because the computer was unavailable...
Glycerols
- 2)Ps/wRBS/LuxR + wRBS/TetA(C)/LuxI, 4 colonies (1 didn't grow)
- 6)Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI, 4 colonies
- 8)Ps/sRBS/LuxR + mRBS/TetA(C)/LuxI, 1 colony
- 9)Pm/sRBS/LuxR + sRBS/TetA(C)/LuxI, 1 colony
- 10)Ps/wRBS/LuxR + sRBS/TetA(C)/LuxI, 2 colonies
- 11)Ps/mRBS/LuxR + sRBS/TetA(C)/LuxI, 4 colonies (2 didn't grow)
Restriction digest with SpeI and PstI : 5µl miniprep + 15µL water + 2x1µl enzyme + 2.5µl B2 + 0.25µl BSA
- PmW/attC
Restriction digest with XbaI and PstI : 5µl miniprep + 15µL water + 2x1µl enzyme + 2.5µl B3 + 0.25µl BSA
- 3x Ps/wRBS/LuxR + wRBS/TetA(C)/LuxI
- 4x Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI
- 1x Ps/sRBS/LuxR + mRBS/TetA(C)/LuxI
- 1x Pm/sRBS/LuxR + sRBS/TetA(C)/LuxI
- 2x Ps/wRBS/LuxR + sRBS/TetA(C)/LuxI
- 2x Ps/mRBS/LuxR + sRBS/TetA(C)/LuxI
- 4x wRBS/LacZα/LuxI
- 4x sRBS/LacZα/LuxI
- LuxI
- 3x pLux/LuxI
Gel electrophoresis
- Ps/wRBS/LuxR + wRBS/TetA(C)/LuxI, colony II
- Ps/wRBS/LuxR + wRBS/TetA(C)/LuxI, colony III
- Ps/wRBS/LuxR + wRBS/TetA(C)/LuxI, colony IV
- Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI, colony I
- Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI, colony II
- Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI, colony III
- Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI, colony IV
- Ps/sRBS/LuxR + mRBS/TetA(C)/LuxI, colony I
- Pm/sRBS/LuxR + sRBS/TetA(C)/LuxI, colony I
- Ps/wRBS/LuxR + sRBS/TetA(C)/LuxI, colony I
- Ps/wRBS/LuxR + sRBS/TetA(C)/LuxI, colony II
- Ps/mRBS/LuxR + sRBS/TetA(C)/LuxI, colony I
- Ps/mRBS/LuxR + sRBS/TetA(C)/LuxI, colony IV
- pLux/LuxI, colony I
- pLux/LuxI, colony II
- pLux/LuxI, colony III
- Ladder 1kb
<a href="/index.php?title=Special:Upload&wpDestFile=Gel1808_1.jpg" class="new" title="Image:Gel1808 1.jpg">Image:Gel1808 1.jpg</a>
Gel electrophoresis
- PmW/attC
- sRBS/LacZα/LuxI, colony I
- sRBS/LacZα/LuxI, colony II
- sRBS/LacZα/LuxI, colony III
- sRBS/LacZα/LuxI, colony IV
- wRBS/LacZα/LuxI, colony I
- wRBS/LacZα/LuxI, colony II
- wRBS/LacZα/LuxI, colony III
- wRBS/LacZα/LuxI, colony IV
- -
- Ladder 1kb
- LuxI
<a href="/index.php?title=Special:Upload&wpDestFile=Gel1808_2.jpg" class="new" title="Image:Gel1808 2.jpg">Image:Gel1808 2.jpg</a>