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Raphaël and Aleksandra
Test population counter (PsS/attC + PBad/Int 4))
2 conditions :

• 1/2 dilution from the overnight culture -> 10h induction, 0,2% arabinose
• 1/50 dilution from the overnight culture -> 10h induction, 0,2% arabinose

-> After 10h, plating with 1/100 and 1/1000 dilutions (there was just few red colonies on the plates, it's impossible to calculate the recombination rate because the constructs are in a high copy plasmid)

Léa

Minipreps

• 2)Ps/wRBS/LuxR + wRBS/TetA(C)/LuxI, 4 colonies (1 didn't grow)
• 6)Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI, 4 colonies
• 8)Ps/sRBS/LuxR + mRBS/TetA(C)/LuxI, 1 colony
• 9)Pm/sRBS/LuxR + sRBS/TetA(C)/LuxI, 1 colony
• 10)Ps/wRBS/LuxR + sRBS/TetA(C)/LuxI, 4 colonies
• 11)Ps/mRBS/LuxR + sRBS/TetA(C)/LuxI, 4 colonies (2 didn't grow)
• wRBS/LacZα/LuxI, 4 colonies
• sRBS/LacZα/LuxI, 4 colonies
• LuxI
• pLux/LuxI, 3 colonies
• PmW/attC, 1 colony

-> We couldn't use the nanodrop because the computer was unavailable...

Glycerols

• 2)Ps/wRBS/LuxR + wRBS/TetA(C)/LuxI, 4 colonies (1 didn't grow)
• 6)Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI, 4 colonies
• 8)Ps/sRBS/LuxR + mRBS/TetA(C)/LuxI, 1 colony
• 9)Pm/sRBS/LuxR + sRBS/TetA(C)/LuxI, 1 colony
• 10)Ps/wRBS/LuxR + sRBS/TetA(C)/LuxI, 2 colonies
• 11)Ps/mRBS/LuxR + sRBS/TetA(C)/LuxI, 4 colonies (2 didn't grow)

Restriction digest with SpeI and PstI : 5µl miniprep + 15µL water + 2x1µl enzyme + 2.5µl B2 + 0.25µl BSA

• PmW/attC

Restriction digest with XbaI and PstI : 5µl miniprep + 15µL water + 2x1µl enzyme + 2.5µl B3 + 0.25µl BSA

• 3x Ps/wRBS/LuxR + wRBS/TetA(C)/LuxI
• 4x Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI
• 1x Ps/sRBS/LuxR + mRBS/TetA(C)/LuxI
• 1x Pm/sRBS/LuxR + sRBS/TetA(C)/LuxI
• 2x Ps/wRBS/LuxR + sRBS/TetA(C)/LuxI
• 2x Ps/mRBS/LuxR + sRBS/TetA(C)/LuxI
• 4x wRBS/LacZα/LuxI
• 4x sRBS/LacZα/LuxI
• LuxI
• 3x pLux/LuxI

Gel electrophoresis

• Ps/wRBS/LuxR + wRBS/TetA(C)/LuxI, colony II
• Ps/wRBS/LuxR + wRBS/TetA(C)/LuxI, colony III
• Ps/wRBS/LuxR + wRBS/TetA(C)/LuxI, colony IV
• Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI, colony I
• Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI, colony II
• Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI, colony III
• Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI, colony IV
• Ps/sRBS/LuxR + mRBS/TetA(C)/LuxI, colony I
• Pm/sRBS/LuxR + sRBS/TetA(C)/LuxI, colony I
• Ps/wRBS/LuxR + sRBS/TetA(C)/LuxI, colony I
• Ps/wRBS/LuxR + sRBS/TetA(C)/LuxI, colony II
• Ps/mRBS/LuxR + sRBS/TetA(C)/LuxI, colony I
• Ps/mRBS/LuxR + sRBS/TetA(C)/LuxI, colony IV
• pLux/LuxI, colony I
• pLux/LuxI, colony II
• pLux/LuxI, colony III
• Ladder 1kb

<a href="/index.php?title=Special:Upload&wpDestFile=Gel1808_1.jpg" class="new" title="Image:Gel1808 1.jpg">Image:Gel1808 1.jpg</a>

Gel electrophoresis

• PmW/attC
• sRBS/LacZα/LuxI, colony I
• sRBS/LacZα/LuxI, colony II
• sRBS/LacZα/LuxI, colony III
• sRBS/LacZα/LuxI, colony IV
• wRBS/LacZα/LuxI, colony I
• wRBS/LacZα/LuxI, colony II
• wRBS/LacZα/LuxI, colony III
• wRBS/LacZα/LuxI, colony IV
• -
• Ladder 1kb
• LuxI

<a href="/index.php?title=Special:Upload&wpDestFile=Gel1808_2.jpg" class="new" title="Image:Gel1808 2.jpg">Image:Gel1808 2.jpg</a>