Team:Freiburg Bioware/BioBricks/capsid coding

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Capsid Coding

<parttable>viral_vectors_aav_capsid</parttable>

Basic Capsidcoding Constructs

Hanna fragen...

ViralBricks for Loop replacement

ViralBricks are short nucleotide sequences derived from the gene of the viral coat proteins VP1-3 where they encode the two major surface exposed loops. Within the loop tip functional peptides are integrated that will be present in viral vectors when the ViralBrick was cloned over single cutting restriction sites into the corresponding capsid coding plasmid.
Different ViralBrick versions:

  • 453 integration site
  • 587 integration site
  • 587 integration site with HSPG knock-out
    • Different functional motifs:
  • Biotinylation Acceptor Peptide (BAP)
  • His-Affinity tag (His)
  • RGD-Motif for Integrin targeting
  • Z34C a antibody binding motif from proein A

    • Versions of the Rep-Vp123_p5-TATA-less

    Versions of the pCMV_Vp123

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