Team:British Columbia/Notebook Biofilm

Protocol

The biofilm sub-team has obtained a growth curve plotting the biofilm growth of S. aureus RN4220 and 8325-4 strains at 3 to 30 hour incubation times. This basic growth curve can be used for comparison with growth curves under conditions where the biofilm is exposed to DspB only, the phage only, or DspB integrated into the phage. RN4220 is a S. aureus strain without a prophage and 8325-4 is a S. aureus strain that has been cured of phage Փ11, Փ12, and Փ13; both strains were selected based on their susceptibility to phage Փ13. The biofilm data collected has additionally been incorporated into the Modelling sub-team simulation to predict the effect of the synthetic phage on Staphylococcus aureus biofilm growth.