Team:Freiburg Bioware
From 2010.igem.org
![virus (3.5MB) virus](http://www.molbiotech.uni-freiburg.de/iGEM/wiki2010/images/8/87/Virus_homepage.gif)
Gene delivery using viral vectors holds great promise for the treatment of acquired and inherited diseases. The human Adeno-Associated Virus (AAV) is a small, non-pathogenic, single-stranded DNA virus gaining increasing attention being both versatile and effective. Taking current knowledge into account, we generated a recombinant, modularized, BioBrick-compatible AAV ‘Virus Construction Kit’. We provide parts for modified capsid proteins, targeting modules, tumor-specific promoters, and prodrug-activating enzymes as well as readily assembled vectors for gene delivery and production of non-replicative virus particles. The viral tropism is altered by N-terminal fusion or by loop replacement of the capsid proteins. Functionality of viruses constructed from our kit was demonstrated by fluorescent protein expression in infected cells and by prodrug-induced killing of tumor cells upon viral delivery of a thymidine kinase. Incorporating multiple layers of safety, we provide a general tool to the growing field of personalized medicine and demonstrate its use in tumor therapy.
![](https://static.igem.org/mediawiki/2010/5/5f/Freiburg10_Virus_Logo_Small.png)
Project Results
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![](http://www.molbiotech.uni-freiburg.de/iGEM/wiki2010/images/b/b2/Freiburg10_Manual-Logo.png)
Virus Construction Kit
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Statistics
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![](https://static.igem.org/mediawiki/2010/5/5f/Freiburg10_Virus_Logo_Small.png)
BioBricks
Modularisation and Modification of the viral tropismn and the effector function resulted in many different BioBricks.
Click here to explore them.
![](https://static.igem.org/mediawiki/2010/0/0d/Freiburg10_Danger_Virus.png)
Biosafety
The aspect of biological safety was considered well and a risk assessment and security profile for the Adeno-associated Virus were created. To hear about this click here.
![](http://www.molbiotech.uni-freiburg.de/iGEM/wiki2010/images/4/40/Freiburg10_Cuckoo_Clock_Competition_button.png)
Notebook
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Sponsors
![Uni Freiburg](https://static.igem.org/mediawiki/2010/b/b8/Freiburg10_Uni-logo.jpg)
![Bioss](https://static.igem.org/mediawiki/2010/e/eb/Freiburg10_sponsors_bioss.jpg)
![kuk.lab](https://static.igem.org/mediawiki/2010/9/9d/Freiburg10_Sponsor_KuK-Lab.png)
![Roche](https://static.igem.org/mediawiki/2010/3/39/Freiburg10_sponsors_Roche.jpg)
![Qiagen](https://static.igem.org/mediawiki/2010/a/a5/Freiburg10_sponsors_Qiagen.jpg)
![Zeiss](https://static.igem.org/mediawiki/2010/7/7f/Freiburg10_Sponsor_Zeiss.jpg)
![Starlab](https://static.igem.org/mediawiki/2010/5/51/Freiburg10_Sponsor_StarLab.gif)
![agilent](https://static.igem.org/mediawiki/2010/f/f1/Freiburg10_Sponsor_Agilent.jpg)
![Fermentas](https://static.igem.org/mediawiki/2010/a/aa/Freiburg10_Sponsor_Fermentas.jpg)
![Hiss](https://static.igem.org/mediawiki/2010/8/89/Freiburg10_Sponsor_HiSS_Diagnostics.jpg)
![peqlab](https://static.igem.org/mediawiki/2010/6/65/Freiburg10_Sponsor_Peqlab.gif)
![Geneart](https://static.igem.org/mediawiki/2010/9/9a/Freiburg10_Sponsor_Geneart.png)
![Eppendorf](https://static.igem.org/mediawiki/2010/d/d2/Freiburg10_Sponsor_eppendorf.jpeg)
![Gilson](https://static.igem.org/mediawiki/2010/b/ba/Freiburg10_Sponsor_Gilson.jpeg)
![GATC](https://static.igem.org/mediawiki/2010/7/74/Freiburg10_Sponsor_GATC.jpg)
![ATG](https://static.igem.org/mediawiki/2010/f/fb/Freiburg10_Sponsor_ATG.jpg)
![Biozym](https://static.igem.org/mediawiki/2010/f/f4/Freiburg10_Sponsor_Biozym.png)
![Avidity](https://static.igem.org/mediawiki/2010/a/ad/Sponsor_Avidity.png)